Recombinant Thermosynechococcus elongatus Lipoprotein signal peptidase (lspA)
Description
Functional Role in Lipoprotein Processing
SPase II cleaves signal peptides from prolipoproteins, enabling their anchoring to bacterial membranes. In T. elongatus, lspA shares conserved residues with homologs in Rickettsia typhi and E. coli, confirming its catalytic role .
Key Findings from Functional Assays
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Globomycin Resistance: Overexpression of T. elongatus lspA in E. coli confers resistance to globomycin (a SPase II inhibitor), validating its activity .
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Genetic Complementation: In E. coli strain Y815 (temperature-sensitive SPase II mutant), T. elongatus lspA restored growth at 42°C, albeit with lower efficiency (~4.7%) compared to E. coli lspA (~21.5%) .
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Substrate Specificity: Preferentially processes lipoproteins amidated on the d-iso-glutamyl residue, as inferred from structural homology with Rickettsia spp. and E. coli SPase II .
Applications and Research Potential
Recombinant T. elongatus lspA offers opportunities in:
Comparative Analysis with Homologs
Product Specs
Note: We will prioritize shipping the format we have in stock. However, if you require a specific format, please indicate your preference in the order notes. We will accommodate your request as best as possible.
Note: Our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please communicate with us in advance as additional fees will apply.
Generally, liquid form has a shelf life of 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: tel:tlr2420
STRING: 197221.tlr2420
