Recombinant Thermotoga neapolitana tRNA pseudouridine synthase A (truA)

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Cat. No.
BT33868
Source
Yeast
Synonyms
truA; CTN_1157; tRNA pseudouridine synthase A; EC 5.4.99.12; tRNA pseudouridine(38-40) synthase; tRNA pseudouridylate synthase I; tRNA-uridine isomerase I
Purity
>85% (SDS-PAGE)
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Cat. No.
BT33868
Source
E.coli
Synonyms
truA; CTN_1157; tRNA pseudouridine synthase A; EC 5.4.99.12; tRNA pseudouridine(38-40) synthase; tRNA pseudouridylate synthase I; tRNA-uridine isomerase I
Purity
>85% (SDS-PAGE)
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Cat. No.
BT33868
Source
E.coli
Synonyms
truA; CTN_1157; tRNA pseudouridine synthase A; EC 5.4.99.12; tRNA pseudouridine(38-40) synthase; tRNA pseudouridylate synthase I; tRNA-uridine isomerase I
Purity
>85% (SDS-PAGE)
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Cat. No.
BT33868
Source
Baculovirus
Synonyms
truA; CTN_1157; tRNA pseudouridine synthase A; EC 5.4.99.12; tRNA pseudouridine(38-40) synthase; tRNA pseudouridylate synthase I; tRNA-uridine isomerase I
Purity
>85% (SDS-PAGE)
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Cat. No.
BT33868
Source
Mammalian cell
Synonyms
truA; CTN_1157; tRNA pseudouridine synthase A; EC 5.4.99.12; tRNA pseudouridine(38-40) synthase; tRNA pseudouridylate synthase I; tRNA-uridine isomerase I
Purity
>85% (SDS-PAGE)
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Description

Recombinant Production and Purification

The enzyme is heterologously expressed in Saccharomyces cerevisiae and purified to >90% homogeneity . Critical production parameters:

ParameterSpecification
Expression HostSaccharomyces cerevisiae
Purification MethodImmobilized metal-affinity chromatography (IMAC) via His tag
StorageLyophilized in PBS pH 7.4 with 50% glycerol; stable at -20°C
Purity>90% (verified by SDS-PAGE)

Applications in Research

The recombinant enzyme is primarily used in:

  • ELISA-based assays for studying tRNA modification mechanisms .

  • Structural biology: Crystallization studies to resolve catalytic mechanisms of pseudouridylation.

  • Biotechnological tooling: Exploration of thermostable RNA-modifying enzymes for industrial processes .

Comparative Analysis with Homologs

T. neapolitana TruA shares 74% DNA homology with Thermotoga maritima , but its recombinant form distinguishes itself through:

FeatureT. neapolitana TruAAquifex aeolicus TruA
Host for ExpressionYeast E. coli (common for homologs)
Thermal StabilityRetains activity after 1h at 80°C Stable up to 95°C
Tag SystemHis tag Variably tagged in commercial kits

Research Gaps and Future Directions

Despite commercial availability, peer-reviewed studies on recombinant T. neapolitana TruA remain sparse. Priorities for future work include:

  • Kinetic profiling under varying pH and temperature conditions.

  • Structural elucidation to compare with mesophilic pseudouridine synthases.

  • Biotechnological integration into synthetic biology platforms for RNA engineering.

Product Specs

Form
Lyophilized powder. We will preferentially ship the available format, but if you have specific requirements, please note them when ordering, and we will accommodate your request.
Lead Time
Delivery times vary by purchasing method and location. Consult your local distributor for specific delivery details. All proteins are shipped with standard blue ice packs. For dry ice shipping, please contact us in advance; additional charges will apply.
Notes
Avoid repeated freezing and thawing. Working aliquots can be stored at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening to collect contents at the bottom. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50% for your reference.
Shelf Life
Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Generally, the liquid form has a 6-month shelf life at -20°C/-80°C, and the lyophilized form has a 12-month shelf life at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during the manufacturing process. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
truA; CTN_1157; tRNA pseudouridine synthase A; EC 5.4.99.12; tRNA pseudouridine(38-40) synthase; tRNA pseudouridylate synthase I; tRNA-uridine isomerase I
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-245
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Thermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NS-E)
Target Names
truA
Target Protein Sequence
MRRVAAIVEY DGSNFFGYQG QPDVRTVQGV IEDALERIFK QRIYTQAAGR TDAGVHANGQ LIAFNCPNDR MTTEDIKNAM NANLPDDVYV KKVFEVPKNF HPRFDVKKRI YHYFIHTSRE KNVFLRKYAW WFPYELDLEA MRKAAKYLEG THDFTSFKTG SDERDPVRTI YRIRILSLGK DMILIRVEGR SFLRRMVRNI VAALVKVGLR QWEPEKLKEV LEARDRSAAA GTAPAHGLYF YKVLF
Uniprot No.
B9K8Q0

Target Background

Function
Forms pseudouridine at positions 38, 39, and 40 in the anticodon stem and loop of transfer RNAs.
Database Links

KEGG: tna:CTN_1157

STRING: 309803.CTN_1157

Protein Families
TRNA pseudouridine synthase TruA family

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