Recombinant Thermotoga petrophila S-adenosylmethionine decarboxylase proenzyme (speH)

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Cat. No.

BT49567

Source

Yeast

Synonyms

speH; Tpet_0276; S-adenosylmethionine decarboxylase proenzyme; AdoMetDC; SAMDC; EC 4.1.1.50) [Cleaved into: S-adenosylmethionine decarboxylase beta chain; S-adenosylmethionine decarboxylase alpha chain]

Purity

>85% (SDS-PAGE)

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Cat. No.

BT49567

Source

E.coli

Synonyms

Purity

>85% (SDS-PAGE)

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Cat. No.

BT49567

Source

E.coli

Synonyms

Purity

>85% (SDS-PAGE)

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Cat. No.

BT49567

Source

Baculovirus

Synonyms

Purity

>85% (SDS-PAGE)

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Cat. No.

BT49567

Source

Mammalian cell

Synonyms

Purity

>85% (SDS-PAGE)

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Description

Introduction

Recombinant Thermotoga petrophila S-adenosylmethionine decarboxylase proenzyme (SpeH) is a pyruvoyl-dependent enzyme critical for polyamine biosynthesis, catalyzing the decarboxylation of S-adenosylmethionine (AdoMet) to produce dcAdoMet, a precursor for spermidine synthesis . Derived from the hyperthermophilic bacterium Thermotoga petrophila, this enzyme exhibits thermostability and functional robustness, making it valuable for industrial and research applications . Its recombinant form, expressed in Escherichia coli, is widely used to study polyamine metabolism and enzyme mechanisms .

Biochemical Properties

Catalytic Mechanism

Converts AdoMet to dcAdoMet via pyruvoyl-mediated decarboxylation, enabling spermidine biosynthesis .
Inhibition by substrate analogs like 5′-deoxy-5′-dimethylthioadenosine (MMTA) via competitive binding .

Thermostability

Inherits thermostability from T. petrophila, retaining activity at high temperatures (optimal growth: 80°C) .

Cofactor and Ion Dependence

Requires autocatalytically generated pyruvoyl cofactor .
Activity modulated by cations (e.g., K⁺, Rb⁺, Ca²⁺ enhance; others inhibit) .

Recombinant Production and Purification

Expression System

Expressed in E. coli BL21(DE3) using plasmid vectors .
Purified via affinity chromatography, yielding >85% purity (SDS-PAGE) .

Biotechnological Uses

Polyamine Research: Essential for studying spermidine/spermine biosynthesis pathways .
Enzyme Engineering: Serves as a model for thermostable decarboxylases in synthetic biology .

Medical Relevance

Potential utility in diagnostic kits (e.g., SARS-CoV-2 detection via engineered polymerases) .

Comparative Analysis with Homologs

**Table 3: SpeH vs. T. maritima AdoMetDC**

Feature	T. petrophila SpeH	T. maritima AdoMetDC
Optimal Temperature	80°C	80–85°C
Active Site	Conserved pyruvoyl cofactor	Similar dimeric architecture
Substrate Specificity	AdoMet	AdoMet and analogs (e.g., MeAdoMet)

Future Directions

Structural Studies: High-resolution crystallography to elucidate substrate-binding dynamics .
Industrial Scale-Up: Optimization for biofuel production and lignin degradation .

Product Specs

Form

Lyophilized powder. We will ship the format we have in stock. If you have special format requirements, please note them when ordering.

Lead Time

Delivery time varies by purchase method and location. Consult your local distributor for specific delivery times. Proteins are shipped with blue ice packs by default. Request dry ice in advance for an extra fee.

Notes

Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.

Shelf Life

Shelf life depends on storage conditions, buffer, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

Tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.

Synonyms

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-62

Protein Length

full length protein

Purity

>85% (SDS-PAGE)

Species

Thermotoga petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995)

Target Names

speH

Target Protein Sequence

MKSLGRHLVA EFYECDKEVL DNVQLIEQEM KQAAYESGAT IVTSTFHRFL PYGVSGVVVI SE

Uniprot No.

A5IJD2

Target Background

Function

Catalyzes the decarboxylation of S-adenosylmethionine to S-adenosylmethioninamine (dcAdoMet), which is the propylamine donor needed to synthesize spermine and spermidine from putrescine.

Database Links

KEGG: tpt:Tpet_0276

STRING: 390874.Tpet_0276

Protein Families

Prokaryotic AdoMetDC family, Type 1 subfamily

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Recombinant Thermotoga petrophila S-adenosylmethionine decarboxylase proenzyme (speH)

Description

Introduction

Biochemical Properties

Catalytic Mechanism

Thermostability

Cofactor and Ion Dependence

Recombinant Production and Purification

Expression System

Biotechnological Uses

Medical Relevance

Comparative Analysis with Homologs

**Table 3: SpeH vs. T. maritima AdoMetDC**

Future Directions

Product Specs

Target Background

Quick Inquiry

Category

Service