Recombinant Thermus thermophilus (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized preparation.
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized preparation.
Lead Time
Delivery times vary depending on the purchasing method and location. Please consult your local distributor for precise delivery estimates.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires advance notice and incurs additional charges.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires advance notice and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to consolidate the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50%, which may serve as a reference.
Shelf Life
Shelf life depends on various factors, including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is essential for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type is determined during the manufacturing process.
The tag type is determined during production. To request a specific tag, please inform us, and we will prioritize its development.
The tag type is determined during production. To request a specific tag, please inform us, and we will prioritize its development.
Synonyms
miaB; TT_C0942; tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase; EC 2.8.4.3,; Dimethylallyl)adenosine tRNA methylthiotransferase MiaB; tRNA-i(6)A37 methylthiotransferase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-465
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
Target Names
miaB
Target Protein Sequence
MPPCYPFLLS WEGWARDPPG RPGGAMRAHI ITYGCQMNEY DSHLVASELV SLGWELVDSV
EEADFVLVNT CAVRGKPVEK VRSLLGQLRK EKERRGLLIG MMGCLAQLDE GQQMAKKFGV
DVLLGPGALT SLPEALKANE RFFDLTFRED VLDYIPPPPK GALSAHVTII RGCNHHCTYC
IVPTTRGPEV SRHPDLILKE IELLKQAGVV EVTLLGQNVN SYGKDQPGFP SFAELLRMVG
GMGIPRVRFL TSHPVNFTDD IIEAIAETPA ICRYIHLPVQ SGSDRVLRRM AREYRRAHYL
ERIRKIREAL PDAVLSTDII VGFPGETEED FQETLSLYDE VGYDQAYMFI YSPRPGTPAY
KHFQDLPREV KVERLMRLIE KQKEWSYRRN LEWVGKTVEV LVRGEAKEEG FVQGHDRGNH
PVLVPASQAP VPGLYQVEIK QATPHLLFGE VVGAEAPAPI PLPVA
Uniprot No.
Target Background
Function
Function: Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), producing 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that recognize codons beginning with uridine.
Database Links
KEGG: tth:TT_C0942
STRING: 262724.TTC0942
Protein Families
Methylthiotransferase family, MiaB subfamily
Subcellular Location
Cytoplasm.
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