Recombinant Transmembrane BAX inhibitor motif-containing protein 4 (tmbi-4)
Description
Production and Purification
Recombinant TMBIM4 is synthesized using E. coli expression systems, ensuring high purity and consistency . Key production details include:
| Parameter | Detail |
|---|---|
| Expression System | E. coli (in vitro) |
| Purity | ≥95% (SDS-PAGE verified) |
| Storage | Lyophilized or liquid form |
This method avoids eukaryotic post-translational modifications, simplifying structural studies while preserving core functional domains .
Anti-Apoptotic Activity
TMBIM4 inhibits apoptosis induced by intrinsic (e.g., Bax overexpression) and extrinsic (e.g., TNFα/Fas ligand) stimuli. It modulates:
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Calcium signaling: Regulates inositol 1,4,5-trisphosphate (IP3)-mediated Ca²⁺ release and capacitative Ca²⁺ entry .
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ER stress response: Retains pro-survival activity under cellular stress .
Interaction Network
Key molecular partners identified via affinity chromatography and STRING-db analysis :
| Interacting Protein | Role | Interaction Score |
|---|---|---|
| PML | Scaffolds PML nuclear bodies | 0.898 |
| SIGMAR1 | Lipid transport regulation | 0.876 |
| ITPR3 | Mediates IP3 calcium release | 0.814 |
Experimental Use Cases
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Apoptosis assays: Used to study Bax-driven cell death in cancer models .
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Calcium flux studies: Employed in IP3 receptor modulation experiments .
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Structural biology: Serves as a template for transmembrane protein crystallization trials .
Limitations
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Lack of native glycosylation in prokaryotic systems may affect ligand binding studies .
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Functional redundancy with paralogs (TMTC1-3) complicates phenotype interpretation .
Evolutionary and Clinical Relevance
Product Specs
Note: While we prioritize shipping the format currently in stock, we can accommodate specific format requests. Please indicate your preference in the order notes, and we will prepare accordingly.
Note: All proteins are shipped with standard blue ice packs. If you require dry ice shipping, please contact us in advance for an additional fee.
Generally, liquid form has a 6-month shelf life at -20°C/-80°C. Lyophilized form maintains a 12-month shelf life at -20°C/-80°C.
