Recombinant Treponema denticola tRNA (Ile)-lysidine synthase (tilS)
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Product Specs
Form
Lyophilized powder
Lead Time
Delivery times vary depending on the purchasing method and location. Please contact your local distributor for specific delivery timelines.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our default glycerol concentration is 50% and can serve as a reference.
Shelf Life
Shelf life depends on various factors including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type is determined during the manufacturing process. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
tilS; TDE_1341; tRNA(Ile)-lysidine synthase; EC 6.3.4.19; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-460
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Treponema denticola (strain ATCC 35405 / CIP 103919 / DSM 14222)
Target Names
tilS
Target Protein Sequence
MCKIYLMAKS FLKDVLLGFS SLCDRTAAPL RLLLAVSGGA DSMAMLSAFL ELKSDINAEI
FVLTVNHNIR PEKETLGDAQ FVLDFCNDKC PCILAEIPKN TVFDEAKNRK TGIEDAARFL
RYNEFEKAAD SLNADYILTA HNKNDNYETV LMRLFQGSEP EALMGISPRR GRFIRPMLNI
SRSEIEEYLK EKNIPWREDA TNLEASYLRN KVRHNLLPVL SICFDGWQSG LDKSLAKIKA
QNDFVIASYK TKKEEWVLDK KEDCCRCKFL FFLSLDEALK LKFLQEGLIL LKGKRRIPYS
VFDDLMKLSD TKKIIFSGGF CIKKEGDEVL LFKAVTEEKT SEVFYSIWID KPCSFDTPAG
NFKALENDDG FFIVHESDKT CGIGPFKPPF CVRSRLFGDE IETSSGSKKS VKKIINEWNI
DYENRNILPI IEEGGVVKGI YGAVFGKKNW YVVGDLGVKR
Uniprot No.
Target Background
Function
This enzyme ligates lysine onto the cytidine at position 34 of the AUA codon-specific tRNA(Ile) containing the anticodon CAU. This ATP-dependent reaction converts cytidine to lysidine, altering the tRNA's amino acid specificity from methionine to isoleucine.
Database Links
KEGG: tde:TDE1341
STRING: 243275.TDE1341
Protein Families
TRNA(Ile)-lysidine synthase family
Subcellular Location
Cytoplasm.
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