Recombinant Trichodesmium erythraeum NAD (P)H-quinone oxidoreductase subunit L (ndhL)
Description
Role in the NDH-1 Complex
The NDH-1 complex in Trichodesmium facilitates electron transfer from NAD(P)H to quinones, coupling this process to proton translocation across membranes. In T. erythraeum, this enzyme is pivotal for:
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Respiratory metabolism: Maintaining redox balance and ATP synthesis .
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Nitrogen fixation: Regulating intracellular oxygen levels to protect nitrogenase activity, as Trichodesmium lacks heterocysts .
Expression and Purification
Recombinant ndhL is synthesized in multiple systems:
| Host System | Purity | Tag | Source |
|---|---|---|---|
| E. coli | ≥85% | N-terminal His | |
| Yeast/Baculovirus | ≥85% | Unspecified | |
| Cell-free expression | ≥85% | None |
Key challenges include maintaining proper folding and solubility, often addressed by optimizing buffer conditions (e.g., Tris-based buffers with glycerol) .
Functional Studies
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Iron limitation: In T. erythraeum, iron scarcity downregulates nifH (nitrogenase) and photosynthetic genes, indirectly affecting NDH-1 activity .
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Oxygen regulation: Computational models propose that NDH-1-mediated Mehler reactions reduce intracellular oxygen, enabling simultaneous carbon and nitrogen fixation .
Applications in Research
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ELISA assays: Used to quantify ndhL expression in environmental or experimental samples .
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Structural studies: Recombinant ndhL aids in elucidating subunit interactions within the NDH-1 complex .
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Biotechnology: Potential role in bioengineered systems for hydrogen production or redox-driven processes .
Comparative Analysis of ndhL Across Organisms
Challenges and Future Directions
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Mechanistic gaps: The precise role of ndhL in oxygen regulation during nitrogen fixation remains unclear .
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Thermodynamic optimization: T. erythraeum thrives in warm (20–34°C) oligotrophic waters, but recombinant ndhL stability at elevated temperatures is unstudied .
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Translational potential: Leveraging ndhL in synthetic biology for biofuel production or bioremediation requires further exploration .
Product Specs
Note: We will prioritize shipping the format we have in stock. However, if you have any specific format requirements, please indicate them in your order. We will prepare the product according to your specifications.
Note: All of our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please communicate with us in advance, as additional charges will apply.
Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C. The shelf life of the lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: ter:Tery_4495
STRING: 203124.Tery_4495
