Recombinant Trichodesmium erythraeum Photosystem Q (B) protein 2 (psbA3)
Description
Production and Purification
This recombinant protein is synthesized using E. coli expression systems, optimized for high yield and stability:
Reconstitution protocols recommend dissolving the protein in sterile water (0.1–1.0 mg/mL) and adding glycerol (5–50%) for long-term storage at -80°C .
Biochemical Stability
Storage and handling guidelines ensure functional integrity:
Functional and Research Applications
This recombinant protein is primarily used to:
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Study PSII structure-function relationships under varying environmental conditions .
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Investigate D1 protein turnover and photodamage repair mechanisms .
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Develop inhibitors targeting photosynthetic electron transport (e.g., herbicides) .
Comparative Homologs
psbA3 homologs exist in other cyanobacteria, with functional variations:
| Species | Protein Name | Key Differences |
|---|---|---|
| Thermosynechococcus elongatus | Photosystem Q(B) protein 3 | Thermostability adaptations |
| Synechococcus sp. | Photosystem II D1 protein 3 | Distinct regulatory regions |
Limitations and Future Directions
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Knowledge Gaps: Structural dynamics of T. erythraeum D1 under high-light or oxidative stress are poorly characterized.
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Technical Challenges: Low solubility without detergents or glycerol .
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Research Opportunities: CRISPR-edited T. erythraeum strains could clarify psbA3’s role in marine nitrogen fixation .
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them in your order. We will accommodate your request if possible.
Note: All proteins are shipped with standard blue ice packs. If you require dry ice shipping, please inform us in advance. Additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: ter:Tery_4763
STRING: 203124.Tery_4763
