Recombinant Uncharacterized response regulatory protein Rv3143/MT3230 (Rv3143, MT3230)

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Cat. No.

BT37246

Source

Yeast

Purity

>85% (SDS-PAGE)

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Cat. No.

BT37246

Source

E.coli

Purity

>85% (SDS-PAGE)

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Cat. No.

BT37246

Source

E.coli

Purity

>85% (SDS-PAGE)

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Cat. No.

BT37246

Source

Baculovirus

Purity

>85% (SDS-PAGE)

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Cat. No.

BT37246

Source

Mammalian cell

Purity

>85% (SDS-PAGE)

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Description

Interaction with Respiratory Complex I

Rv3143 directly interacts with NuoD, a core subunit of the type I NADH dehydrogenase (NDH-1) complex, as demonstrated by:

Interaction Evidence	Methodology	Source
Co-purification with NuoD	Affinity pulldown + mass spectrometry
Crosslinking via BS3 reagent	Kojak software analysis
In vitro binding (GST/His-tagged proteins)	Western blotting

This interaction modulates electron transport chain activity, particularly under conditions affecting membrane potential .

Phenotypic Effects of Rv3143 Inactivation

Gene knockout studies in M. tuberculosis (Δrv3143) and M. smegmatis (Δmsmeg_2064) revealed:

Respiratory Chain Dysregulation

Increased reduction of 2,3,5-triphenyltetrazolium chloride (TTC), indicating a hyper-reduced cytosolic environment .
Hypersensitivity to valinomycin (3× higher MIC90 in Δrv3143 vs. wild-type), which disrupts membrane potential .
Upregulation of nitrate respiration genes (narGHIJ, nirBD) under standard growth conditions .

Stress Response Deficits

Impaired survival under hypoxia in CRISPRi/dCas9-mediated ndh-silenced strains .
Reduced oxygen consumption during hypoxic adaptation in double mutants (Δmsmeg_2064 ndh CRISPRi/dCas9) .

Gene Silencing and Complementation

Knockout construction: Homologous recombination using p2Nil/pGOAL17 vectors .
Complementation: Restored wild-type phenotypes via pMV306/pKW08Lx plasmids .

Transcriptional Profiling

RNA sequencing of Δrv3143 M. tuberculosis showed:

Upregulated genes: narK2 (nitrite extrusion), moaE3 (molybdenum cofactor biosynthesis).
Downregulated genes: fdxA (ferredoxin), ctaD (cytochrome c oxidase) .

Implications for Tuberculosis Research

Rv3143’s role in maintaining respiratory chain efficiency highlights its potential as a drug target. Key insights include:

Therapeutic vulnerability: Dual targeting of NDH-1 (via Rv3143) and NDH-2 could disrupt M. tuberculosis energy metabolism .
Hypoxia adaptation: Rv3143-deficient strains show compromised survival in low-oxygen environments, relevant to latent TB .

Product Specs

Form

Lyophilized powder. We will preferentially ship the format we have in stock. If you have special format requirements, please note them when ordering, and we will fulfill your request.

Lead Time

Delivery times vary based on purchasing method and location. Please consult your local distributor for specific delivery times. All proteins are shipped with standard blue ice packs by default. For dry ice shipping, please contact us in advance; extra fees will apply.

Notes

Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening to collect contents at the bottom. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.

Shelf Life

Shelf life depends on several factors, including storage conditions, buffer components, storage temperature, and protein stability. Generally, the liquid form has a shelf life of 6 months at -20°C/-80°C, while the lyophilized form has a shelf life of 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

The tag type will be determined during the manufacturing process. If you require a specific tag type, please inform us, and we will prioritize developing it.

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

2-133

Protein Length

Full Length of Mature Protein

Purity

>85% (SDS-PAGE)

Target Names

Rv3143, MT3230

Target Protein Sequence

PDSSTALRI LVYSDNVQTR ERVMRALGKR LHPDLPDLTY VEVATGPMVI RQMDRGGIDL AILDGEATPT GGMGIAKQLK DELASCPPIL VLTGRPDDTW LASWSRAEAA VPHPVDPIVL GRTVLSLLRA PAH

Uniprot No.

P95183

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Recombinant Uncharacterized response regulatory protein Rv3143/MT3230 (Rv3143, MT3230)

Description

Interaction with Respiratory Complex I

Phenotypic Effects of Rv3143 Inactivation

Respiratory Chain Dysregulation

Stress Response Deficits

Gene Silencing and Complementation

Transcriptional Profiling

Implications for Tuberculosis Research

Product Specs

Quick Inquiry

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Service