Recombinant UPF0266 membrane protein YPO1755/y2554/YP_1637 (YPO1755, y2554, YP_1637)
Description
Protein Overview
Recombinant UPF0266 membrane protein YPO1755/y2554/YP_1637 (UniProt ID: Q8ZFF7) is a 153-amino acid polypeptide expressed in heterologous systems such as E. coli, yeast, baculovirus, or mammalian cells . It belongs to the UPF0266 protein family, which includes uncharacterized membrane proteins conserved in Gram-negative bacteria.
Production Methods
The protein is commercially produced in multiple expression systems, each offering distinct advantages:
| Host System | Yield | Post-Translational Modifications | Turnaround Time |
|---|---|---|---|
| E. coli | High | Limited | 2–3 weeks |
| Yeast | Moderate | Basic glycosylation | 3–4 weeks |
| Baculovirus (Insect) | Low-Moderate | Complex modifications (e.g., phosphorylation) | 6–8 weeks |
| Mammalian Cells | Low | Human-like modifications | 8–10 weeks |
E. coli remains the most cost-effective option for bulk production, while mammalian systems are preferred for functional studies requiring native-like folding .
Research Applications
This recombinant protein is primarily used in:
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ELISA Development: Detecting anti-Yersinia antibodies in serum samples .
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Structural Biology: Studying membrane protein folding via X-ray crystallography .
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Pathogenicity Studies: Investigating Y. pestis host-cell interactions.
Quality Control Data
Critical benchmarks from recent batches:
| Parameter | Specification |
|---|---|
| Endotoxin Levels | <1.0 EU/µg (Limulus Amebocyte Lysate test) |
| Aggregation Analysis | <5% by dynamic light scattering |
| Functional Activity | Confirmed via ligand-binding assays |
Supplier Landscape
Major vendors include Creative BioMart ($1,496/50 µg) , American Scientific, and Lifeome. Pricing varies by expression system, with mammalian-cell-derived versions costing 3–5× more than E. coli-produced equivalents .
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized preparation.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires advance notice and incurs additional charges.
Note: While the tag type is determined during production, please specify your desired tag type for preferential development.
Target Background
KEGG: ype:YPO1755
STRING: 187410.y2554
