Recombinant UPF0397 protein PA0141 (PA0141)

Introduction to UPF0397 Protein PA0141

UPF0397 protein PA0141, also known simply as PA0141, is a full-length protein derived from Phytoplasma australiense. The protein belongs to the UPF0397 family, which consists of proteins with currently uncharacterized functions. The recombinant form of this protein has been developed for research purposes, typically expressed with tags such as His-tag to facilitate purification and experimental manipulation. The protein consists of 193 amino acids and is commercially available in various forms for research applications .

Expression Systems

The recombinant UPF0397 protein PA0141 is typically expressed in Escherichia coli (E. coli) expression systems. This bacterial expression system allows for efficient production of the protein in sufficient quantities for research and commercial purposes. The use of E. coli for protein expression represents a standard approach in molecular biology for producing recombinant proteins due to its well-characterized genetics, rapid growth, and high protein yields .

Reconstitution Protocol

For optimal use, the following reconstitution protocol is recommended:

1. Briefly centrifuge the vial prior to opening to bring the contents to the bottom

2. Reconstitute the protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL

3. Add glycerol to a final concentration of 5-50% (50% is commonly recommended)

4. Aliquot for long-term storage at -20°C/-80°C

Research Applications

While the specific functions of PA0141 remain to be fully elucidated, the availability of recombinant forms of this protein facilitates various research applications:

1. Structural studies to determine the three-dimensional configuration of the protein

2. Functional assays to investigate potential enzymatic activities

3. Protein-protein interaction studies to identify binding partners

4. Development of antibodies against the protein for detection purposes

5. Investigation of potential roles in cellular processes

Relationship to Polyphosphate Kinase Activity

Based on the limited information available in the search results, there appears to be a potential relationship between PA0141 and polyphosphate kinase activity. According to source , a related protein has been associated with "polyphosphate kinase 2, PA0141" functional domain and may be involved in phosphorus metabolic processes . This suggests a possible role for PA0141 in phosphorus metabolism, though further research would be needed to confirm this function specifically for the Phytoplasma australiense PA0141 protein.