Recombinant Uracil phosphoribosyltransferase (upp)

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Cat. No.

BT55408

Source

Yeast

Synonyms

upp; spyM18_0443; Uracil phosphoribosyltransferase; EC 2.4.2.9; UMP pyrophosphorylase; UPRTase

Purity

>85% (SDS-PAGE)

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Cat. No.

BT55408

Source

E.coli

Synonyms

upp; spyM18_0443; Uracil phosphoribosyltransferase; EC 2.4.2.9; UMP pyrophosphorylase; UPRTase

Purity

>85% (SDS-PAGE)

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Cat. No.

BT55408

Source

E.coli

Synonyms

upp; spyM18_0443; Uracil phosphoribosyltransferase; EC 2.4.2.9; UMP pyrophosphorylase; UPRTase

Purity

>85% (SDS-PAGE)

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Cat. No.

BT55408

Source

Baculovirus

Synonyms

upp; spyM18_0443; Uracil phosphoribosyltransferase; EC 2.4.2.9; UMP pyrophosphorylase; UPRTase

Purity

>85% (SDS-PAGE)

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Cat. No.

BT55408

Source

Mammalian cell

Synonyms

upp; spyM18_0443; Uracil phosphoribosyltransferase; EC 2.4.2.9; UMP pyrophosphorylase; UPRTase

Purity

>85% (SDS-PAGE)

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Description

Table 1: Kinetic Parameters of Recombinant UPP

Source	Substrate	K<sub>M</sub> (µM)	V<sub>max</sub> (U mg<sup>−1</sup>)	k<sub>cat</sub> (s<sup>−1</sup>)
M. tuberculosis	PRPP	2.9 ± 0.1	1.54 ± 0.02	0.57 ± 0.01
M. tuberculosis	Uracil	2.3 ± 0.1	1.60 ± 0.04	0.59 ± 0.01
B. caldolyticus	Uracil	0.5 mM	18% cellular protein yield	Not reported

Functional Insights

pH Dependence: MtUPP activity peaks at pH 7.8, with pK values of 6.6 and 8.2 for catalytic residues .
Thermostability: B. caldolyticus UPP retains activity at 70°C, outperforming B. subtilis homologs .
GTP Modulation: Unlike E. coli UPP, MtUPP activity remains unaffected by GTP, suggesting evolutionary divergence in regulatory mechanisms .

Phenotypic Effects of upp Knockouts

Lactococcus lactis Δupp mutants lose UPP activity, excrete uracil, and exhibit 5-fluorouracil (FU) resistance .
Secondary mutations in thymidine phosphorylase or kinase restore FU resistance by blocking alternative uracil metabolism .

Table 2: Stability and Inhibitor Responses

Organism	Thermal Stability (50°C)	FU Resistance	Uracil Excretion (µg/ml)
L. lactis wild-type	Not applicable	Sensitive	0.17 ± 0.01
L. lactis Δupp	Not applicable	Resistant	0.35 ± 0.01
B. caldolyticus UPP	Stable for 15 min	Not tested	Not applicable

Biotechnological Uses

Metabolic Engineering: Δupp strains serve as auxotrophic hosts for plasmid selection in L. lactis .
Enzyme Immobilization: B. caldolyticus UPP’s thermostability makes it suitable for industrial biocatalysis .

Therapeutic Potential

Antimicrobial Targets: MtUPP is essential for M. tuberculosis survival, validated by gene knockout studies .
Suicide Gene Therapy: Protozoan UPP converts 5-FU into toxic metabolites, a strategy explored for cancer treatment .

Table 3: Microbial vs. Eukaryotic UPP Features

Feature	Microbial UPP	Eukaryotic UPP
Catalytic Activity	High (e.g., MtUPP: 0.59 s⁻¹)	Weak or absent (human UPP)
PRPP Binding Motif	Conserved (Arg77, Arg102)	Partially conserved
Therapeutic Relevance	Drug target for infections	Limited due to low activity

Challenges and Future Directions

Structural Optimization: Engineering UPP for enhanced stability or altered substrate specificity could expand industrial applications .
Host Compatibility: Heterologous expression in E. coli often requires codon optimization to improve yields .

Product Specs

Form

Lyophilized powder. We will ship the format we have in stock. If you have special format requirements, please note them when ordering, and we will fulfill your request.

Lead Time

Delivery time varies depending on purchase method and location. Consult your local distributor for specific delivery times. All proteins are shipped with standard blue ice packs. For dry ice shipment, contact us in advance; extra fees apply.

Notes

Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening to collect contents at the bottom. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.

Shelf Life

Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Liquid form shelf life is generally 6 months at -20°C/-80°C. Lyophilized form shelf life is generally 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

Tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.

Synonyms

upp; spyM18_0443; Uracil phosphoribosyltransferase; EC 2.4.2.9; UMP pyrophosphorylase; UPRTase

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-209

Protein Length

full length protein

Purity

>85% (SDS-PAGE)

Species

Streptococcus pyogenes serotype M18 (strain MGAS8232)

Target Names

upp

Target Protein Sequence

MGKCQVISHP LIQHKLSILR RQTTSTKDFR ELVNEIAMLM GYEVSRDLPL EDVDIQTPVS KTVQKQLAGK KLAIVPILRA GIGMVDGLLS LVPAAKVGHI GMYRNEETLE PVEYLVKLPE DINQRQIFLV DPMLATGGSA ILAVDSLKKR GAANIKFVCL VAAPEGVKKL QEAHPDIDIF TAALDDHLNE HGYIVPGLGD AGDRLFGTK

Uniprot No.

P67402

Target Background

Function

Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate.

Database Links

KEGG: spm:spyM18_0443

Protein Families

UPRTase family

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Recombinant Uracil phosphoribosyltransferase (upp)

Description

Table 1: Kinetic Parameters of Recombinant UPP

Functional Insights

Phenotypic Effects of upp Knockouts

Table 2: Stability and Inhibitor Responses

Biotechnological Uses

Therapeutic Potential

Table 3: Microbial vs. Eukaryotic UPP Features

Challenges and Future Directions

Product Specs

Target Background

Quick Inquiry

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Service