Recombinant Variovorax paradoxus Peptide chain release factor 1 (prfA)
Description
Definition and Context
Recombinant Peptide Chain Release Factor 1 (prfA) is a protein involved in terminating translation during bacterial protein synthesis. While the term "prfA" is commonly associated with Wigglesworthia glossinidia brevipalpis (based on available data), no direct evidence exists in the provided sources linking prfA to Variovorax paradoxus. The search results primarily focus on Variovorax paradoxus enzymes like N-acyl-d-amino acid amidohydrolase (N-d-AAase), glycine oxidase (VpGO), and GFP expression systems, with no mention of prfA in this species .
Potential Confusion in Species Attribution
The only documented recombinant prfA in the provided sources originates from Wigglesworthia glossinidia brevipalpis, not Variovorax paradoxus. Key details include:
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Product Code: CSB-YP813370WAN
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Immunogen Species: Wigglesworthia glossinidia brevipalpis
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Sequence: MKFSFEKNYA LVQKLKKLKN RYKEIKIILS DPFSAKKQKN FFLLSKEYSK LNILNKYLKS LESINNTIES TKTMLLDPEM HDLAKNEINI YKNKKKLIEK KIILYLIPNE DEYICNCFIE IRSGTGGKEA SLFVRDLCRM YIKYSEIKNW KTDIINYSNS EHGGYKEVII KICNKNAYNK LKFESGGHRV QRIPETESQG .
| Property | Value |
|---|---|
| Molecular Weight | Not explicitly stated in sources |
| Purity | >85% (SDS-PAGE) |
| Storage Conditions | Liquid: 6 months at -20°C/-80°C; Lyophilized: 12 months at -20°C/-80°C |
Functional Insights from Analogous Systems
While Variovorax paradoxus prfA is not described, insights into bacterial release factors can be extrapolated from Listeria monocytogenes PrfA studies :
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Role: PrfA is a transcriptional regulator controlling virulence genes in Listeria. Mutations in PrfA disrupt RNA polymerase binding and transcription initiation .
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Structural Domains:
Research Gaps and Recommendations
The absence of Variovorax paradoxus prfA data in the literature highlights critical gaps:
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Species-Specific Function: No evidence links prfA to Variovorax translation machinery.
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Operon Structure: Unlike N-d-AAase and VpGO, which form operons in Variovorax , prfA’s regulatory context remains undefined.
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Experimental Validation: Studies on Variovorax prfA would require cloning, expression, and functional assays (e.g., EMSA, in vitro transcription).
Product Specs
Target Background
KEGG: vap:Vapar_4206
STRING: 543728.Vapar_4206
