Recombinant Vibrio vulnificus Ribosomal RNA small subunit methyltransferase B (rsmB)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order remarks for customized preparation.
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order remarks for customized preparation.
Lead Time
Delivery times vary depending on the purchasing method and location. Please contact your local distributor for precise delivery estimates.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50% and can serve as a guideline.
Shelf Life
Shelf life depends on several factors: storage conditions, buffer composition, temperature, and the protein's inherent stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized formulations have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquot for multiple uses to prevent repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing.
The tag type is determined during production. If you require a specific tag, please inform us; we will prioritize its development.
The tag type is determined during production. If you require a specific tag, please inform us; we will prioritize its development.
Synonyms
rsmB; rrmB; VV3227; Ribosomal RNA small subunit methyltransferase B; EC 2.1.1.176; 16S rRNA m5C967 methyltransferase; rRNA; cytosine-C(5)-)-methyltransferase RsmB
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-426
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Vibrio vulnificus (strain YJ016)
Target Names
rsmB
Target Protein Sequence
MNVRAAAANV LYQVVDRGNS LSTALPEAQQ SIRPRDHALL QEICYGALRY LPRLESIAGK
LMDKPLKGKQ RVFHHLILVG LYQLGHMRIP AHAAVGETVE GAQALKGPRL RGLINAVLRN
YQREQQALDE FSVSHNAGKY VHPSWLLKIL QDAYPEQWQT IVEANNNKAP MWLRVNHAHH
DRDEYLQLLK NANIDSTLHS EAMDAIKLAA PCDVHSLPGF EQGWVSVQDA AAQLSFNYLQ
PKDGELILDC CAAPGGKTAH ILERVSAREV VALDCDEQRL KRVTENLKRL NLQAKVICGD
ARNPEQWWQG EQFDRILLDA PCSATGVIRR HPDIKWLRRA EDITALAQLQ SEIFDAMWAQ
LKPGGTLVYA TCSITPMENV EQVKAFLART PAAQLVGSER ENPGRQILPG EEDMDGFYYA
VLVKQA
Uniprot No.
Target Background
Function
Specifically methylates cytosine at position 967 (m5C967) of 16S rRNA.
Database Links
KEGG: vvy:VV3227
Protein Families
Class I-like SAM-binding methyltransferase superfamily, RsmB/NOP family
Subcellular Location
Cytoplasm.
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