Recombinant Xanthomonas campestris pv. campestris Porphobilinogen deaminase (hemC)

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Cat. No.
BT38176
Source
Yeast
Synonyms
hemC; xcc-b100_0685; Porphobilinogen deaminase; PBG; EC 2.5.1.61; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT38176
Source
E.coli
Synonyms
hemC; xcc-b100_0685; Porphobilinogen deaminase; PBG; EC 2.5.1.61; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT38176
Source
E.coli
Synonyms
hemC; xcc-b100_0685; Porphobilinogen deaminase; PBG; EC 2.5.1.61; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT38176
Source
Baculovirus
Synonyms
hemC; xcc-b100_0685; Porphobilinogen deaminase; PBG; EC 2.5.1.61; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT38176
Source
Mammalian cell
Synonyms
hemC; xcc-b100_0685; Porphobilinogen deaminase; PBG; EC 2.5.1.61; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase
Purity
>85% (SDS-PAGE)
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Description

Overview of Recombinant Xanthomonas campestris pv. campestris Porphobilinogen Deaminase (HemC)

Porphobilinogen deaminase (HemC) is a critical enzyme in the heme biosynthesis pathway, catalyzing the conversion of porphobilinogen to hydroxymethylbilane. In Xanthomonas campestris pv. campestris (Xcc), HemC is encoded by the hemC gene and plays a role in bacterial metabolism and virulence. Recombinant HemC refers to the enzyme produced via heterologous expression systems (e.g., Escherichia coli) for functional and structural studies.

Functional Role of HemC in Xcc

HemC is essential for synthesizing tetrapyrroles, including heme and vitamin B12, which are vital for:

  • Cytochrome function: Electron transport and energy metabolism .

  • Stress tolerance: Oxidative stress resistance via heme-dependent catalases .

  • Pathogenicity: Indirectly supporting virulence mechanisms through metabolic robustness .

Disruption of hemC in Xcc would impair heme biosynthesis, leading to auxotrophy and reduced survival under oxidative stress .

3.1. Genetic and Regulatory Context

  • Gene localization: The hemC gene is part of the heme biosynthesis operon, often co-regulated with other hem genes .

  • Regulatory networks: HemC expression may intersect with methyltransferase HemK, which modulates virulence and nutrient utilization .

3.3. Recombinant Production and Applications

  • Expression systems: hemC is cloned into plasmids (e.g., pET vectors) under inducible promoters (e.g., T7) for overexpression in E. coli .

  • Purification: Typically involves affinity chromatography (e.g., His-tag) and size-exclusion chromatography .

  • Applications:

    • Enzyme kinetics: Studying substrate specificity and inhibitor screening .

    • Antimicrobial targets: Designing inhibitors to disrupt heme biosynthesis in Xcc .

Comparative Genomic Insights

  • Conservation: The hemC gene is conserved across Xanthomonas species, including X. citri and X. oryzae, with >90% sequence identity .

  • Mobilome association: No mobile genetic elements (e.g., transposons) are linked to hemC, suggesting stable inheritance .

Challenges and Research Gaps

  • Functional redundancy: Potential overlap with other tetrapyrrole biosynthesis pathways in Xcc remains unexplored .

  • Structural data: No crystallographic data for Xcc HemC are available, limiting mechanistic studies .

Product Specs

Form
Lyophilized powder. We will ship the in-stock format preferentially. If you have specific format requirements, please note them when ordering.
Lead Time
Delivery times vary by purchase method and location. Consult your local distributor for specific delivery times. All proteins ship with standard blue ice packs. Request dry ice shipment in advance (extra fees apply).
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
hemC; xcc-b100_0685; Porphobilinogen deaminase; PBG; EC 2.5.1.61; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-304
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Xanthomonas campestris pv. campestris (strain B100)
Target Names
hemC
Target Protein Sequence
MTTLRIATRK SPLALWQSEH VATALRQHHP GLEVVLVPMS TRGDEVLDRS LAAIGGKGLF LKELELAMLR GEADCAVHSL KDVPMELDAP FVLPAILERG DPADALVSNL YATLQALPLG ARVGTSSLRR QAQLRAARPD LELIDLRGNV NTRLAKLDNG GYDAIVLACA GLQRLGLEAR ITARLDAPEW LPAPAQGAVA VECRGDDARI HDLLAVLDAG RTRACVEAER AMNRALHGSC HVPVAAFARW EGEDLFLQGM VGSASDGRLI HAEAHGSPDA TEDLGRRVAD GLFEKGAAQL LAEL
Uniprot No.
B0RNI9

Target Background

Function
Forms hydroxymethylbilane pre-uroporphyrinogen by tetrapolymerizing the monopyrrole PBG in several steps.
Database Links

KEGG: xca:xcc-b100_0685

Protein Families
HMBS family

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