Recombinant Xanthomonas oryzae pv. oryzae Probable ubiquinone biosynthesis protein UbiB (ubiB)

Introduction to Recombinant Xanthomonas oryzae pv. oryzae Probable Ubiquinone Biosynthesis Protein UbiB (ubiB)

Recombinant Xanthomonas oryzae pv. oryzae Probable ubiquinone biosynthesis protein UbiB (ubiB) is a recombinant protein derived from the bacterium Xanthomonas oryzae pv. oryzae, a pathogen responsible for bacterial blight in rice . This protein is involved in the ubiquinone biosynthesis pathway, which is crucial for the electron transport chain and energy production in bacteria. The recombinant form of this protein is produced through genetic engineering techniques, allowing for its use in various biochemical and biotechnological applications.

Characteristics of Recombinant UbiB Protein

The recombinant UbiB protein from Xanthomonas oryzae pv. oryzae is characterized by its specific amino acid sequence and structural features. The protein is produced in a recombinant form, typically in a host organism like Escherichia coli, and is purified for use in research or diagnostic applications.

• Amino Acid Sequence: The protein sequence includes a specific arrangement of amino acids that define its structure and function. The sequence provided for this protein includes motifs typical of ubiquinone biosynthesis enzymes .

• Storage and Handling: The recombinant protein is typically stored in a Tris-based buffer with 50% glycerol at -20°C to maintain stability. Repeated freezing and thawing should be avoided .

Biological Role of UbiB in Xanthomonas oryzae pv. oryzae

UbiB is part of the ubiquinone biosynthesis pathway, which is essential for bacterial energy metabolism. Ubiquinone (also known as Coenzyme Q) plays a critical role in the electron transport chain, facilitating the generation of ATP during oxidative phosphorylation.

Research Findings and Applications

Research on recombinant UbiB from Xanthomonas oryzae pv. oryzae is limited, but its study contributes to understanding bacterial metabolism and pathogenicity. The protein can be used in various biochemical assays to study ubiquinone biosynthesis and its role in bacterial physiology.

• Biochemical Assays: Recombinant UbiB can be used in enzyme assays to study the ubiquinone biosynthesis pathway. This helps in understanding how modifications in this pathway might affect bacterial metabolism and pathogenicity.

• Diagnostic Tools: The protein could potentially be used in developing diagnostic tools for detecting Xanthomonas oryzae pv. oryzae infections, although this would require further development.

Data and Tables

While specific data tables for recombinant UbiB from Xanthomonas oryzae pv. oryzae are not readily available, the following table summarizes key characteristics of the protein: