Recombinant Xenopus laevis DNA replication licensing factor mcm7-B (mcm7-b), partial
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized preparation.
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized preparation.
Lead Time
Delivery times vary depending on the purchase method and location. Please contact your local distributor for precise delivery estimates.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires advance notice and incurs additional charges.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires advance notice and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to consolidate the contents. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50%, which can serve as a guideline.
Shelf Life
Shelf life depends on storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is essential for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
mcm7-b; cdc47-2DNA replication licensing factor mcm7-B; EC 3.6.4.12; CDC47 homolog B; CDC47-2p; Minichromosome maintenance protein 7-B; xMCM7-B
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Protein Length
Partial
Purity
>85% (SDS-PAGE)
Species
Xenopus laevis (African clawed frog)
Target Names
mcm7-b
Uniprot No.
Target Background
Function
This protein is a component of the mcm2-7 complex (minichromosome maintenance complex), a replicative helicase crucial for the initiation and elongation of DNA replication ('once per cell cycle') in eukaryotic cells. The mcm2-7 ring's ATPase active sites are formed via interactions between neighboring subunits, with a conserved arginine finger motif playing a crucial structural role. While all six ATPase sites contribute to helicase activity, their individual contributions likely vary. The presence of maternal and zygotic forms of mcm3 and mcm6 suggests the use of distinct mcm2-7 complexes during different developmental stages.
Gene References Into Functions
- The mcm2-7 complex associates with Cdc45 and GINS to form the CMG (Cdc45-MCM-GINS) complex. PMID: 21282109
Database Links
KEGG: xla:380414
UniGene: Xl.4048
Protein Families
MCM family
Subcellular Location
Nucleus. Chromosome.
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