Recombinant Xenopus laevis Protein slowmo homolog 1 (slmo1)

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Description

Introduction to Recombinant Xenopus laevis Protein slowmo homolog 1 (slmo1)

Recombinant Xenopus laevis Protein slowmo homolog 1 (slmo1) is a protein of interest in molecular biology, particularly in studies involving protein-protein interactions and cellular processes. While specific details about this protein might be limited, related proteins like SLMO1 in other organisms have been studied extensively. For instance, SLMO1 in humans is known to interact with TRIAP1, a protein involved in mitochondrial phospholipid metabolism . This article will delve into the available information on slmo1 and related proteins, focusing on their structure, function, and interactions.

SLMO1 interacts with TRIAP1 via a hydrophobic stripe, which is crucial for their complex formation. Mutagenesis studies have identified key residues at the interface, such as V36 and L49 in SLMO1, which are essential for maintaining the interaction . The TRIAP1-SLMO1 complex plays a role in supplying phosphatidic acid to the inner mitochondrial membrane, highlighting its importance in mitochondrial phospholipid metabolism.

Key ResiduesProteinRole in Interaction
V36, L49SLMO1Essential for complex formation
F41TRIAP1Key interfacial contact

Research Findings and Implications

Research on SLMO1 and related proteins has implications for understanding mitochondrial function and phospholipid metabolism. The conservation of structural features across species suggests a conserved mechanism of action. Further studies on recombinant Xenopus laevis Protein slowmo homolog 1 could provide insights into its specific role in cellular processes and potential applications in biotechnology or biomedical research.

Product Specs

Form
Lyophilized powder
Note: We will prioritize shipping the format currently in stock. However, please specify any format requirements in your order notes, and we will accommodate your request to the best of our ability.
Lead Time
Delivery times vary depending on the purchasing method and location. Please contact your local distributor for precise delivery estimates.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Before opening, briefly centrifuge the vial to collect the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and storing in aliquots at -20°C/-80°C. Our standard glycerol concentration is 50% and may be used as a reference.
Shelf Life
Shelf life depends on various factors, including storage conditions, buffer composition, temperature, and the protein's inherent stability.
Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C. Lyophilized formulations have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is essential for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing.
Tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its use in production.
Synonyms
prelid3a; slmo1; PRELI domain containing protein 3A; Protein slowmo homolog 1
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-172
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Xenopus laevis (African clawed frog)
Target Names
prelid3a
Target Protein Sequence
MRIWSSEHVF SHPWDTVIKA AMRKYPNPMN PCVVGVDVVD RNLDSQGRLH SQRLLCTEWG LPSLVRAILG TSRTLTYIKE HSVVDPVEKK MVLCSTNISL TNLVSVDERL VYTPHPENPE QTVLTQEAII TVKGVSLSSY LEGLMASTIS SNARKGWDAI EWIIQNSEST VS
Uniprot No.

Target Background

Function
In vitro, the triap1:prelid3a complex facilitates phosphatidic acid (PA) transfer between liposomes, likely functioning as a PA transporter across the mitochondrial intermembrane space. PA import is crucial for cardiolipin (CL) synthesis within the mitochondrial inner membrane.
Database Links

KEGG: xla:444585

UniGene: Xl.29441

Protein Families
Slowmo family
Subcellular Location
Mitochondrion.

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