Recombinant Xenopus tropicalis Coiled-coil domain-containing protein 167 (ccdc167)
Description
Gene and Protein Overview
CCDC167 is encoded by the ccdc167 gene (Entrez Gene ID: 100125155) in Xenopus tropicalis. Key characteristics include:
The protein’s coiled-coil domain suggests roles in structural organization or scaffold formation, potentially influencing mitotic processes or signaling pathways .
Production and Characteristics
Recombinant CCDC167 is typically expressed in E. coli with an N-terminal His-tag for purification. Key production parameters include:
The recombinant protein’s stability and solubility are optimized for biochemical assays, including ELISA, Western blot, or interaction studies .
Functional Associations and Interactions
CCDC167 interacts with diverse cellular components and pathways, as inferred from functional databases:
Key Functional Partners
| Protein | Function |
|---|---|
| ARFGEF3 | Regulates insulin granule biogenesis in pancreatic β-cells |
| CHMP7 | Involved in nuclear envelope sealing during mitosis |
| SAC3D1 | Promotes centrosome duplication and mitotic progression |
| LRRTM4 | Excitatory synaptogenesis in neuronal systems |
These interactions suggest CCDC167 may regulate organelle dynamics, cell division, or synaptic plasticity.
Pathway Involvement
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Cell Cycle Regulation: APC-CDH1 complex-mediated mitotic progression.
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Immune Response: Interferon (IFN)-α/β signaling and MHC class I antigen presentation.
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Ubiquitination: Metabolism of ubiquitin-like modifiers (e.g., SUMO).
Research Applications
Recombinant CCDC167 is utilized in studies probing its role in disease and basic biology:
ELISA Applications
| Parameter | Detail |
|---|---|
| Product | Xenopus CCDC167 ELISA kit (50 µg vial) |
| Sensitivity | Optimized for detecting endogenous or recombinant CCDC167 |
| Applications | Quantifying protein levels in lysates or conditioned media |
Cancer Research Insights
While human CCDC167 is implicated in breast cancer progression, the recombinant Xenopus protein may serve as a model for studying:
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Proliferation: Knockdown reduces colony formation in breast cancer cell lines (e.g., MCF-7).
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Drug Response: Chemotherapeutic agents (e.g., doxorubicin) downregulate CCDC167 expression.
Product Specs
Note: We prioritize shipping the format currently in stock. However, if you have a specific format requirement, please indicate it when placing your order, and we will fulfill your request.
Note: All of our proteins are shipped with standard blue ice packs. If dry ice shipping is required, please communicate with us in advance as additional charges will apply.
Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C. The shelf life of the lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: xtr:100125155
UniGene: Str.52526
