Recombinant Xenopus tropicalis Cytoplasmic tRNA 2-thiolation protein 1 (ctu1)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your preferred format in order notes for customized preparation.
Note: While we prioritize shipping the format currently in stock, please specify your preferred format in order notes for customized preparation.
Lead Time
Delivery times vary depending on the purchasing method and location. Please contact your local distributor for precise delivery estimates.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50%, provided as a reference.
Shelf Life
Shelf life depends on various factors including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is essential for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing.
The tag type is determined during production. If a specific tag type is required, please inform us, and we will prioritize its development.
The tag type is determined during production. If a specific tag type is required, please inform us, and we will prioritize its development.
Synonyms
ctu1; atpbd3; ncs6Cytoplasmic tRNA 2-thiolation protein 1; EC 2.7.7.-; ATP-binding domain-containing protein 3; Cytoplasmic tRNA adenylyltransferase 1
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-341
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Target Names
ctu1
Target Protein Sequence
MPVNCSSCEE RRAVLRRPKT GHSLCKDCFF HAFEEEIHHT IVSAKLFNPG EKVGIGASGG
KDSTVLAHVL KVLNERYAYG LDLILVSVDE GISGYRDDSL ETVKRNQQQY ELPLKIVSYQ
ELYGWTMDQI VKQVGLKNNC TFCGVFRRQA LDRGAMMLGV NKICTGHNAD DIAETVLMNF
LRGDIARLRR CTSITTGSEG AIPRCKPLKY AYEKEIVLYA YFKKLDYFST ECIYSPNAYR
GHARAFLKDL EAIRPSSIMD IIHSGENLSV KEDVRMPVQG TCTRCGYISS QSLCKACVLL
EGLNRGLPKL GIGKHHKLHH KLLSQEPLSE QEERKLKAVD F
Uniprot No.
Target Background
Function
This protein plays a crucial role in the 2-thiolation of mcm(5)S(2)U at tRNA wobble positions in tRNA(Lys), tRNA(Glu), and tRNA(Gln). It directly binds tRNAs and likely catalyzes tRNA adenylation, a necessary intermediate for 2-thiolation. The precise mechanism, including whether it functions as a sulfurtransferase transferring sulfur from thiocarboxylated urm1 to the uridine at the tRNA wobble position, remains to be fully elucidated.
Database Links
Protein Families
TtcA family, CTU1/NCS6/ATPBD3 subfamily
Subcellular Location
Cytoplasm.
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