Recombinant Xenopus tropicalis Eukaryotic translation initiation factor 2 subunit 1 (eif2s1)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: While we will prioritize shipping the format currently in stock, please specify any format requirements in your order notes for fulfillment.
Note: While we will prioritize shipping the format currently in stock, please specify any format requirements in your order notes for fulfillment.
Lead Time
Delivery times vary depending on the purchase method and location. Please contact your local distributor for precise delivery estimates.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50% and can serve as a reference.
Shelf Life
Shelf life depends on various factors including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is crucial for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type is determined during manufacturing.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
eif2s1; eif2a; TGas059e16.1Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
2-315
Protein Length
Full Length of Mature Protein
Purity
>85% (SDS-PAGE)
Species
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Target Names
Target Protein Sequence
PALSCRFYQ HKFPEVDDVV MVNVRSIAEM GAYVSLLEYN NIEGMILLSE LSRRRIRSIN
KLIRIGRNEC VVVIRVDKDK GYIDLSKRRV SPEEALKCED KFTKSKTVYS ILRHVAEVLE
YTKDEQLESL YQRTAWVFDE KYKKPGYGAY DAFKHAVSDP DVLDGLDLNE DERRVLIDNI
NRRLTPQAVK IRADIEVACY GYEGIDAVKD ALRAGLSCST ENMPIKINLI APPRYVMTTT
TLERTEGLSV LNQAMSVIKE RIEEKRGVFN VQMEPKVVTD TDETELARQL ERLEKENAEV
DGDDDAEEME AKTED
Uniprot No.
Target Background
Function
Eukaryotic translation initiation factor 2 subunit 1 (eIF2S1) plays a crucial role in the early stages of protein synthesis. It forms a ternary complex with GTP and initiator tRNA, which subsequently binds to a 40S ribosomal subunit followed by mRNA binding to create a 43S pre-initiation complex. The 60S ribosomal subunit joins to form the 80S initiation complex, preceded by GTP hydrolysis from bound eIF-2 and the release of an eIF-2-GDP binary complex. eIF-2 recycling and subsequent initiation rounds require GDP-to-GTP exchange, a reaction catalyzed by eIF-2B. eIF2S1/eIF-2α is a key component of the integrated stress response (ISR), essential for adapting to various stresses. Phosphorylation by stress-sensing protein kinases in response to stress converts eIF2S1/eIF-2α into a global protein synthesis inhibitor, attenuating cap-dependent translation while preferentially initiating translation of ISR-specific mRNAs, including transcriptional activators ATF4 and QRICH1.
Database Links
KEGG: xtr:448087
UniGene: Str.6631
Protein Families
EIF-2-alpha family
Subcellular Location
Cytoplasm, Stress granule.
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