Recombinant Xenopus tropicalis Piwi-like protein 2 (piwil2), partial

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Cat. No.

BT31300

Source

Yeast

Synonyms

piwil2; Piwi-like protein 2; EC 3.1.26.-

Purity

>85% (SDS-PAGE)

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Cat. No.

BT31300

Source

E.coli

Synonyms

piwil2; Piwi-like protein 2; EC 3.1.26.-

Purity

>85% (SDS-PAGE)

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Cat. No.

BT31300

Source

E.coli

Synonyms

piwil2; Piwi-like protein 2; EC 3.1.26.-

Purity

>85% (SDS-PAGE)

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Cat. No.

BT31300

Source

Baculovirus

Synonyms

piwil2; Piwi-like protein 2; EC 3.1.26.-

Purity

>85% (SDS-PAGE)

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Cat. No.

BT31300

Source

Mammalian cell

Synonyms

piwil2; Piwi-like protein 2; EC 3.1.26.-

Purity

>85% (SDS-PAGE)

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Description

Production and Purification

Expression System

Host: Escherichia coli (BL21-Rosetta or BL21-Star strains) .
Vector: pET15b plasmid with N-terminal His-tag for affinity purification .

Key Roles in Germline Biology

Transposon Silencing: Associates with piRNAs to repress retrotransposons during meiosis, safeguarding genomic integrity .
Ping-Pong Cycle: Acts as a "slicer-competent" endoribonuclease, cleaving primary piRNAs to generate secondary piRNAs that load onto Piwil4 (MIWI2) .
Spermatogenesis: Essential for spermatogonial stem cell self-renewal and meiotic differentiation .

Regulatory Functions

Circadian Rhythm Modulation: Enhances stability and activity of ARNTL/BMAL1 and CLOCK proteins.
piRNA Biogenesis: Required for nuclear localization of Piwil4 and antisense piRNA association.

Experimental Applications

In Vitro Studies: Used to investigate piRNA-Piwi interactions and transposon repression mechanisms .
Antibody Production: Immunogens for generating specific antisera .

Comparative Analysis with Other Piwi Proteins

Feature	X. tropicalis Piwil2	Mouse Mili	Drosophila Ago3
Expression	Oocytes, early embryos	Testis, oocytes	Germline, somatic
piRNA Binding	Primary/secondary	Primary	Secondary
Slicer Activity	Yes	Yes	No
Transposon Targets	LINEs, SINEs	LINE1	Gypsy
Data synthesized from

Research Advancements and Challenges

Key Findings

Oocyte Localization: Piwil2 is enriched in early-stage oocytes, colocalizing with translational repressors like CPEB .
Dynamic piRNA Populations: Associates with piRNAs averaging 29–34 nt, longer than those in zebrafish or mammals .

Technical Limitations

Partial Sequence: Lack of full-length protein limits structural studies on domain interactions .
Bacterial Expression: Absence of post-translational modifications may alter functional assays .

Product Specs

Form

Lyophilized powder. We will preferentially ship the available format. If you have specific format requirements, please note them when ordering.

Lead Time

Delivery time varies by purchase method and location. Consult local distributors for specific delivery times. Proteins are shipped with blue ice packs by default. Request dry ice in advance for an extra fee.

Notes

Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.

Shelf Life

Shelf life depends on storage conditions, buffer, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

Tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.

Synonyms

piwil2; Piwi-like protein 2; EC 3.1.26.-

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Protein Length

Partial

Purity

>85% (SDS-PAGE)

Species

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Target Names

piwil2

Uniprot No.

A8KBF3

Target Background

Function

Endoribonuclease piwil2 is crucial for spermatogenesis by repressing transposable elements, ensuring germline integrity. It's essential for meiotic differentiation of spermatocytes, germ cell differentiation, and spermatogonial stem cell self-renewal. Piwil2 participates in the piRNA metabolic process, which represses transposable elements during meiosis via piRNA-Piwi protein complexes, leading to transposon methylation and repression. In piRNA biosynthesis, piwil2 is key in the ping-pong amplification cycle. As a 'slicer-competent' endoribonuclease, it cleaves primary piRNAs, which load onto 'slicer-incompetent' piwil4. Piwil2 slicing creates a pre-miRNA intermediate (processed into mature piRNAs) and a degraded 16-nucleotide by-product. It's required for piwil4/miwi2 nuclear localization and association with antisense secondary piRNAs. Piwil2 also represses circadian rhythms by promoting ARNTL/BMAL1 and CLOCK stability and activity.

Gene References Into Functions

1. Xiwi interacts with meiotic microtubules and translational regulators in an RNA-dependent manner, while Xenopus tudor interacts with Xiwi independently of RNA. (PMID: 19713941)

Database Links

KEGG: xtr:100127654

STRING: 8364.ENSXETP00000023096

UniGene: Str.43305

Protein Families

Argonaute family, Piwi subfamily

Subcellular Location

Cytoplasm. Nucleus.

Tissue Specificity

Expressed in oocytes, testis and liver (at protein level).

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Recombinant Xenopus tropicalis Piwi-like protein 2 (piwil2), partial

Description

Production and Purification

Expression System

Key Roles in Germline Biology

Regulatory Functions

Experimental Applications

Comparative Analysis with Other Piwi Proteins

Research Advancements and Challenges

Key Findings

Technical Limitations

Product Specs

Target Background

Quick Inquiry

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