Recombinant Xenopus tropicalis RWD domain-containing protein 3 (rwdd3)
Description
Definition and Biological Role
The RWD Domain-Containing Protein 3 (RWDD3) is a conserved protein involved in cellular signaling pathways. In humans, RWDD3 regulates NF-κB transcription factor activity and hypoxia-inducible factor-1α (HIF-1α) signaling, playing roles in inflammation and oxygen response . Its ortholog in Xenopus tropicalis (a diploid frog model organism) shares structural and functional homology, enabling its use in developmental genetics and disease modeling .
Recombinant Protein Production
Recombinant RWDD3 proteins are synthesized using heterologous expression systems to study protein function, interactions, and therapeutic potential. Key production details include:
| Expression Host | Tag | Sequence Coverage | Purity | Applications |
|---|---|---|---|---|
| Yeast | His-Tag | AA 1–265 | >90% | ELISA, Western Blotting |
| E. coli | His-Tag | Full-Length (AA 1–457) | >90% | Functional Studies, SDS-PAGE |
| HEK-293 Cells | Strep-Tag | AA 1–267 | >70–80% | ELISA, Functional Studies |
Post-Translational Modifications
Recombinant proteins retain eukaryotic modifications (e.g., glycosylation) when expressed in yeast or mammalian systems, enhancing their utility in functional assays .
Applications in Research
-
Signaling Pathway Studies: RWDD3 modulates NF-κB and HIF-1α signaling, making it a target for investigating inflammation and hypoxia .
-
Cancer and Regenerative Biology: X. tropicalis RWDD3 orthologs are implicated in organogenesis and regeneration, aligning with its use in amphibian model systems .
-
Therapeutic Development: Recombinant proteins facilitate drug screening and antibody production for potential anti-inflammatory therapies .
Research Findings
Product Specs
Target Background
KEGG: xtr:550029
UniGene: Str.17439
