Recombinant Xenopus tropicalis Sepiapterin reductase (spr)
Shipped with Ice Packs 
In Stock 
Description
Production and Purification
The recombinant protein is expressed in heterologous systems for research use:
-
Expression Systems:
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Purification: Affinity chromatography using a C-terminal His-tag ensures >90% purity .
Table 2: Key Production Metrics
| Parameter | Detail |
|---|---|
| Host System | Yeast (Saccharomyces cerevisiae) |
| Tag | His-tag (N-terminal) |
| Purity | >90% (SDS-PAGE) |
| Applications | ELISA, enzymatic assays |
| Sources: |
Catalytic Roles
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BH4 Biosynthesis: Converts 6-pyruvoyltetrahydropterin to BH4 via NADPH-dependent reduction .
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Redox Cycling: Mediates ROS generation via quinone redox cycling, independent of its sepiapterin reduction activity .
Mutagenesis Studies
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D257H Mutation: Abolishes sepiapterin reduction but minimally affects redox cycling, suggesting distinct mechanisms .
Comparative Analysis with Mammalian SPR
| Feature | Xenopus tropicalis SPR | Human SPR |
|---|---|---|
| Amino Acids | 261 | 261–262 |
| Redox Cycling | Observed | Confirmed |
| Tissue Distribution | Broad (brain, liver) | Ubiquitous |
Applications in Disease Research
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Neurological Disorders: Used to model BH4 deficiencies linked to dopa-responsive dystonia and Parkinson’s disease .
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Oxidative Stress: Mechanistic studies on ROS-mediated lung injury and cancer .
Research Limitations
Product Specs
Form
Lyophilized powder. We will preferentially ship the available format. If you have specific format requirements, please note them when ordering.
Lead Time
Delivery times vary by purchase method and location. Consult your local distributor for specific delivery information. Proteins are shipped with blue ice packs by default. For dry ice shipping, contact us in advance; extra fees apply.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
spr; Sepiapterin reductase; SPR; EC 1.1.1.153
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-261
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Target Names
spr
Target Protein Sequence
MAATGALGSV LCVLTGASRG FGRTLAHLLC PRLLPGSTLL LVSRTEEALK GLAGELAHKY
PGVRVRWEAA DLGTSEGVSA AVRAAGELQV GAAQKLLIIN NAGSIGDVSK MFVDFSDPKE
VTDYMMFNVS SPLCLTASLL KTFPRRPDLQ RVVVNVSSLA ALQPFKSWAL YCSGKAARDM
IFRVLAEEEK DVRVLNYAPG PLDTDMHVVA RTQTADPELR RFLMDRKEKG KMVDIQVSAK
KMLDLLEADA YKSGDHIDFF D
Uniprot No.
Target Background
Function
Catalyzes the final one or two reductions in tetrahydrobiopterin biosynthesis to produce 5,6,7,8-tetrahydrobiopterin.
Database Links
Protein Families
Sepiapterin reductase family
Subcellular Location
Cytoplasm.
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