Recombinant Xenopus tropicalis Transmembrane protein 211 (tmem211)
Product Specs
Please note: We will prioritize shipping the format currently in stock. However, if you have specific format requirements, kindly indicate them in your order notes, and we will fulfill your request to the best of our ability.
Our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please contact us in advance as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. Lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Target Background
Q&A
Basic Research Questions
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Why is Xenopus tropicalis preferred over Xenopus laevis for recombinant tmem211 studies?
Xenopus tropicalis offers several advantages over X. laevis for recombinant protein studies:
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True diploid genome (unlike the allotetraploid X. laevis), simplifying genetic manipulation and analysis
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Higher conservation of gene synteny with the human genome, enhancing translational relevance
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Shorter generation time, facilitating multigenerational experiments and transgenic line creation
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Simpler genome that was sequenced before X. laevis, providing more robust genomic resources
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More amenable to targeted mutagenesis approaches using modern gene editing technologies
These advantages make X. tropicalis particularly valuable for studying transmembrane proteins like tmem211 where genetic manipulation and cross-species comparisons are crucial.
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What expression systems are optimal for producing recombinant Xenopus tropicalis tmem211?
For recombinant expression of X. tropicalis tmem211, E. coli-based systems have been successfully employed with specific considerations for membrane proteins . The methodology includes:
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Using expression vectors containing N-terminal 10xHis tags to facilitate purification
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Optimizing codon usage for bacterial expression
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Culturing in Tris/PBS-based buffer systems (pH 8.0)
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Supplementing with 6% trehalose as a stabilizing agent
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Implementing either liquid formulation or lyophilization for stable storage
Alternative expression systems like insect cells or mammalian cells may provide better post-translational modifications but require different optimization parameters.
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How should recombinant tmem211 be stored and handled to maintain activity?
Optimal handling of recombinant tmem211 requires:
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Storage at -20°C/-80°C upon receipt
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Aliquoting for multiple use to avoid repeated freeze-thaw cycles
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For lyophilized protein: 12-month shelf life at -20°C/-80°C
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For liquid formulation: 6-month shelf life at -20°C/-80°C
Stability can be improved by adding glycerol (10-15%) to prevent freeze-damage to the transmembrane regions during storage.
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What are common applications for recombinant X. tropicalis tmem211 in basic research?
Basic research applications include:
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Antibody production and validation
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Protein-protein interaction studies
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Structural analysis of transmembrane domains
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Comparative studies with human TMEM211 homologs
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Development of functional assays for membrane transport or signaling
These applications provide foundation knowledge before proceeding to more complex in vivo studies.
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