Recombinant Xylella fastidiosa Succinyl-CoA ligase [ADP-forming] subunit beta (sucC)

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Cat. No.
BT48107
Source
Yeast
Synonyms
sucC; XfasM23_2037; Succinate--CoA ligase [ADP-forming] subunit beta; EC 6.2.1.5; Succinyl-CoA synthetase subunit beta; SCS-beta
Purity
>85% (SDS-PAGE)
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Cat. No.
BT48107
Source
E.coli
Synonyms
sucC; XfasM23_2037; Succinate--CoA ligase [ADP-forming] subunit beta; EC 6.2.1.5; Succinyl-CoA synthetase subunit beta; SCS-beta
Purity
>85% (SDS-PAGE)
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Cat. No.
BT48107
Source
E.coli
Synonyms
sucC; XfasM23_2037; Succinate--CoA ligase [ADP-forming] subunit beta; EC 6.2.1.5; Succinyl-CoA synthetase subunit beta; SCS-beta
Purity
>85% (SDS-PAGE)
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Cat. No.
BT48107
Source
Baculovirus
Synonyms
sucC; XfasM23_2037; Succinate--CoA ligase [ADP-forming] subunit beta; EC 6.2.1.5; Succinyl-CoA synthetase subunit beta; SCS-beta
Purity
>85% (SDS-PAGE)
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Cat. No.
BT48107
Source
Mammalian cell
Synonyms
sucC; XfasM23_2037; Succinate--CoA ligase [ADP-forming] subunit beta; EC 6.2.1.5; Succinyl-CoA synthetase subunit beta; SCS-beta
Purity
>85% (SDS-PAGE)
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Description

Definition and Enzymatic Role

Recombinant Xylella fastidiosa Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) is the beta subunit of the succinyl-CoA synthetase (SCS) enzyme complex, produced via recombinant DNA technology. This subunit dictates nucleotide specificity (ADP/ATP) and binds succinate, while the alpha subunit (sucD) binds coenzyme A and phosphate.

In the tricarboxylic acid (TCA) cycle, SCS catalyzes the reversible conversion of succinyl-CoA to succinate, coupled with ATP/GTP synthesis . This represents the sole substrate-level phosphorylation step in the TCA cycle, making it essential for energy production and metabolite biosynthesis.

Metabolic Studies

  • TCA Cycle Dynamics: Recombinant sucC enables mechanistic studies of energy metabolism in X. fastidiosa, which colonizes plant xylem and insect vectors .

  • Biofilm and Virulence Link: While not directly studied in X. fastidiosa, homologous sucC/SCS systems in other pathogens influence biofilm formation and stress adaptation .

Intersubspecific Recombination

Natural competence and recombination events in X. fastidiosa subspecies (e.g., fastidiosa, multiplex) could theoretically alter sucC functionality. For example:

  • Subspecies multiplex exhibits lower recombination frequencies compared to fastidiosa, potentially affecting metabolic gene evolution .

  • Type I restriction-modification systems in X. fastidiosa may influence horizontal gene transfer of sucC-like alleles .

Evolutionary and Pathogenic Implications

  • Commensal vs. Parasitic Behavior: Enzymatic processing of exopolysaccharides (EPS) in X. fastidiosa biofilms attenuates virulence in some hosts . While sucC is not directly linked to EPS modulation, its metabolic role may indirectly affect pathogenicity.

  • Host-Specific Adaptation: Strains with higher sucC activity could optimize energy production for survival in nutrient-limited xylem environments .

Future Directions

  • Targeted Mutagenesis: Knockout studies could clarify sucC’s role in X. fastidiosa’s dual lifestyle (plant pathogen vs. insect commensal).

  • Therapeutic Potential: Inhibiting sucC might disrupt energy metabolism, offering a strategy to control diseases like Pierce’s disease in grapes .

Product Specs

Form
Lyophilized powder. We will preferentially ship the available format. For specific format requirements, please note them during order placement.
Lead Time
Delivery time varies by purchase method and location. Consult local distributors for specific delivery times. Proteins are shipped with blue ice packs by default. For dry ice shipment, contact us in advance (extra fees apply).
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you require a specific tag, please inform us and we will prioritize its development.
Synonyms
sucC; XfasM23_2037; Succinate--CoA ligase [ADP-forming] subunit beta; EC 6.2.1.5; Succinyl-CoA synthetase subunit beta; SCS-beta
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-387
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Xylella fastidiosa (strain M23)
Target Names
sucC
Target Protein Sequence
MNFHEYQAKQ LFAEYGIPVP AGRIASSADE AVTAAKSLGN GPWMVKAQIH AGGRGKAGGV KFCKTTDEVK QAAATMLGTK MATYQSAGVA LPVNLVLVTE AGEITKELYL SVLVDRGTRS ITYIASSEGG VDIEHVAAET PEKIQTLNVD FVEGLQPYQG RDIGFHLGLE AKQVNQLSKI MISLYQLFND KDLSLIELNP LAILSNGDLY ALDGKINSDD NATFRHKELA AMRDKTQEDE TEVLASENDL NYVTMDGNIG CMVNGAGLAM ATMDVIKLNG GEPANFLDVG GGATKERVTT AFKLILSSNK VKAIFVNIFG GIVRCDMIAE GIIAAVKEVG VKVPVIVRLE GTNVDAGKQL LATSGLAIIP ADDINDGAKK AVAAVTV
Uniprot No.
B2I9R4

Target Background

Function
Succinyl-CoA synthetase, part of the citric acid cycle (TCA), couples succinyl-CoA hydrolysis to ATP or GTP synthesis, representing the only substrate-level phosphorylation step in the TCA. The beta subunit determines nucleotide specificity and binds succinate, while the alpha subunit binds coenzyme A and phosphate.
Database Links

KEGG: xfn:XfasM23_2037

Protein Families
Succinate/malate CoA ligase beta subunit family

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