Recombinant Yarrowia lipolytica Peroxisomal biogenesis factor 2 (PEX2)

Introduction to Recombinant Yarrowia lipolytica Peroxisomal Biogenesis Factor 2 (PEX2)

Recombinant Yarrowia lipolytica Peroxisomal Biogenesis Factor 2 (PEX2) is a protein crucial for the assembly and function of peroxisomes in the yeast Yarrowia lipolytica. Peroxisomes are organelles involved in various metabolic processes, including fatty acid oxidation, amino acid metabolism, and detoxification of reactive oxygen species. PEX2 is part of a family of proteins known as peroxins, encoded by PEX genes, which are essential for peroxisome biogenesis and maintenance.

Function of PEX2 in Peroxisomal Biogenesis

PEX2 is an integral membrane protein of peroxisomes and plays a significant role in the import of peroxisomal matrix proteins. It is involved in the early steps of peroxisome assembly and is crucial for maintaining the structural integrity of peroxisomes. In Yarrowia lipolytica, mutations affecting PEX2 or other peroxins can lead to impaired peroxisomal function, resulting in the inability to utilize certain carbon sources like oleic acid .

Research Findings on PEX2

Research on PEX2 in Yarrowia lipolytica highlights its importance in peroxisomal biogenesis. Studies have shown that mutations in pex19, another peroxin, lead to reduced levels of PEX2, indicating a potential interaction between these proteins in maintaining peroxisomal membrane stability . The interaction between PEX2 and other peroxins like PEX19 suggests a complex network of proteins involved in peroxisome assembly and function.

Recombinant Expression of PEX2

Recombinant expression of PEX2 involves the production of this protein in a controlled environment, often using bacterial or yeast expression systems. This approach allows for the large-scale production of PEX2 for research purposes, enabling detailed studies of its structure and function. Recombinant PEX2 can be used to investigate protein-protein interactions, enzymatic activities, and its role in peroxisomal biogenesis.

Data and Tables

While specific data tables for recombinant Yarrowia lipolytica PEX2 are not readily available in the provided sources, research findings typically involve biochemical assays, subcellular localization studies, and interaction analyses. For example, studies might include:

References

1. Yarrowia lipolytica Pex20p, Saccharomyces cerevisiae Pex18p... .

2. Yarrowia lipolytica Cells Mutant for the Peroxisomal Peroxin Pex19p... .

3. Recombinant Full Length Yarrowia Lipolytica Peroxisomal... .