Recombinant Yersinia pseudotuberculosis serotype O:3 Acyl carrier protein (acpP)
Description
Functional Role in Bacterial Physiology
AcpP is essential for shuttling acyl groups during fatty acid biosynthesis. In Y. pseudotuberculosis, it acts as a cofactor for enzymes like OpgGH, which synthesize osmoregulated periplasmic glucans (OPGs) critical for motility, biofilm formation, and virulence . ACP’s role extends to:
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Lipid A biosynthesis: Required for cell envelope integrity and stress adaptation .
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Virulence regulation: Indirectly modulates pathogenicity through metabolic pathways linked to OPG production .
Research Findings and Applications
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Biochemical Activity: The Aas protein (containing AcpP) catalyzes the transfer of acyl groups from acyl-ACP to phospholipids, influencing membrane dynamics .
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Genetic Context: The aas gene is part of a conserved operon in Y. pseudotuberculosis, with homologs in Y. pestis and other Enterobacteriaceae .
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Experimental Use: Recombinant AcpP is utilized to study bacterial lipid metabolism and screen antimicrobial agents targeting fatty acid synthesis .
Expression and Purification Protocol
The recombinant protein is produced via the following steps :
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Cloning: Full-length aas gene insertion into an E. coli expression vector.
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Induction: IPTG-driven expression under optimized conditions.
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Purification: Nickel-affinity chromatography leveraging the N-terminal His tag.
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Quality Control: SDS-PAGE and Western blot validation for purity and identity.
Reconstitution Note: For long-term storage, reconstitution in sterile water (0.1–1.0 mg/mL) with 50% glycerol is recommended .
Key Research Insights
Product Specs
Target Background
KEGG: ypy:YPK_1687
