Recombinant Yersinia pseudotuberculosis serotype O:3 Enolase-phosphatase E1 (mtnC)

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Cat. No.
BT53681
Source
Yeast
Synonyms
mtnC; YPK_3320; Enolase-phosphatase E1; EC 3.1.3.77; 2,3-diketo-5-methylthio-1-phosphopentane phosphatase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT53681
Source
E.coli
Synonyms
mtnC; YPK_3320; Enolase-phosphatase E1; EC 3.1.3.77; 2,3-diketo-5-methylthio-1-phosphopentane phosphatase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT53681
Source
E.coli
Synonyms
mtnC; YPK_3320; Enolase-phosphatase E1; EC 3.1.3.77; 2,3-diketo-5-methylthio-1-phosphopentane phosphatase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT53681
Source
Baculovirus
Synonyms
mtnC; YPK_3320; Enolase-phosphatase E1; EC 3.1.3.77; 2,3-diketo-5-methylthio-1-phosphopentane phosphatase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT53681
Source
Mammalian cell
Synonyms
mtnC; YPK_3320; Enolase-phosphatase E1; EC 3.1.3.77; 2,3-diketo-5-methylthio-1-phosphopentane phosphatase
Purity
>85% (SDS-PAGE)
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Description

Overview of Enolase-Phosphatase E1 (MtnC)

Enolase-phosphatase E1, encoded by the mtnC gene, is a bifunctional enzyme involved in the methionine salvage pathway. It catalyzes two sequential reactions:

  1. Enolase activity: Conversion of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) to 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).

  2. Phosphatase activity: Dephosphorylation of DHK-MTPene to 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTP) .

In Yersinia pseudotuberculosis serotype O:3, this enzyme is critical for metabolizing methylthioadenosine (MTA), a byproduct of polyamine biosynthesis, to regenerate methionine—a process essential for bacterial survival under sulfur-limiting conditions .

Genomic Context and Functional Significance

The mtnC gene is part of the mtnKABD operon in Y. pseudotuberculosis, which includes:

GeneFunction
mtnK5-Methylthioribose kinase
mtnADehydratase
mtnBEnolase-phosphatase E1 (MtnC)
mtnDAcireductone dioxygenase

This operon enables the bacterium to utilize MTA as a sulfur source, enhancing its adaptability in host environments . Comparative genomic studies reveal that mtnC is conserved across Yersinia species but exhibits unique regulatory elements in Y. pseudotuberculosis serotype O:3, potentially linked to its pathogenicity .

Research Gaps and Future Directions

Existing studies on Y. pseudotuberculosis focus on virulence factors (e.g., Yops, LcrV) and vaccine development , with limited emphasis on metabolic enzymes like MtnC. Key unanswered questions include:

  • Structural Characterization: No crystal structure of Y. pseudotuberculosis MtnC is available.

  • Role in Virulence: Whether MtnC contributes to pathogenicity or survival in host tissues remains unexplored.

  • Therapeutic Targeting: Potential as a drug target due to its role in methionine salvage .

Comparative Analysis with Homologs

FeatureY. pseudotuberculosis MtnCE. coli MtnC
Molecular Weight~25 kDa (predicted)~23 kDa
Operon StructuremtnKABDmtnKABD
Subcellular LocalizationCytoplasmicCytoplasmic
Catalytic EfficiencyNot characterizedKm=0.8 μMK_m = 0.8 \ \mu M for DK-MTP-1-P

Product Specs

Form
Lyophilized powder. We will ship the format we have in stock. If you have special format requirements, please note them when ordering.
Lead Time
Delivery time varies by purchase method and location. Consult local distributors for specific delivery times. Proteins are shipped with blue ice packs. Request dry ice in advance (extra fees apply).
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Default glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you have a specific tag type requirement, please inform us.
Synonyms
mtnC; YPK_3320; Enolase-phosphatase E1; EC 3.1.3.77; 2,3-diketo-5-methylthio-1-phosphopentane phosphatase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-229
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Yersinia pseudotuberculosis serotype O:3 (strain YPIII)
Target Names
mtnC
Target Protein Sequence
MIQAIVTDIE GTTTDIRFVH QVLFPYARER LTPFLRAHQQ DDDIAALLVD LRREIAQPDA DIETLITVLH GFMDEDRKST VLKAIQGIIW RTGYLQADFR GHVYPEVAQQ LADWHQQGLK LYVYSSGSVA AQKLLFGYSD AGDLCPLFSG YFDTHVGAKR DVSAYQKIAN QLGIAPQALL FLSDIRQELD AAQLAGWHTC QLIRDLPDND SAHPQVNRFD QIVLSLFTE
Uniprot No.
B1JIK5

Target Background

Function
This bifunctional enzyme catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) to 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).
Database Links

KEGG: ypy:YPK_3320

Protein Families
HAD-like hydrolase superfamily, MasA/MtnC family

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