Recombinant Yersinia pseudotuberculosis serotype O:3 Queuine tRNA-ribosyltransferase (tgt)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder. Note: We will prioritize shipping the format we have in stock. If you have specific format requirements, please note them when ordering, and we will fulfill your request.
Lead Time
Delivery times vary depending on the purchase method and location. Please consult your local distributor for specific delivery times. Note: All proteins are shipped with standard blue ice packs. If you require dry ice shipping, please contact us in advance, as additional fees will apply.
Notes
Avoid repeated freezing and thawing. Working aliquots can be stored at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening to collect the contents at the bottom. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50% for your reference.
Shelf Life
Shelf life depends on several factors, including storage conditions, buffer components, storage temperature, and protein stability. Generally, the liquid form has a shelf life of 6 months at -20°C/-80°C, while the lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during the manufacturing process. If you require a specific tag type, please inform us, and we will prioritize developing it.
Synonyms
tgt; YPK_3264; Queuine tRNA-ribosyltransferase; EC 2.4.2.29; Guanine insertion enzyme; tRNA-guanine transglycosylase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-374
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Yersinia pseudotuberculosis serotype O:3 (strain YPIII)
Target Names
tgt
Target Protein Sequence
MKYELQKTDG RARRGRLVFE RGVVETPAFM PVGTYGTVKG MTPEEVKETG AQILLGNTFH
LWLRPGQEIM KLHGDLHDFM QWHGPILTDS GGFQVFSLGA MRKIKEEGVH FKNPINGDSV
FLSPEKSMEI QYDLGSDIVM IFDECTPYPA DWDYAKRSME MSLRWAARSR QRFDELNNKN
ALFGIIQGGV YEDLRDVSVK GLVDIGFDGY AVGGLAVGEP KEDMHRILEH VCPQIPEDKP
RYLMGVGKPE DLVEGVRRGI DMFDCVMPTR NARNGHLFVT DGVVKIRNAK HKDDTATLDE
HCDCYTCRHY SRAYLHHLDR CNEILGARLN TIHNLRYYQR LMAGLRQAIE EGKLEHFVED
FYGRIGKPVP PLNV
Uniprot No.
Target Background
Function
Queuine tRNA-ribosyltransferase (tgt) catalyzes the replacement of guanine (G) with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His, and -Tyr). This occurs via a double-displacement mechanism. The active site nucleophile attacks the C1' of nucleotide 34, removing the guanine base from the RNA and forming a covalent enzyme-RNA intermediate. The active site proton acceptor deprotonates PreQ1, enabling it to attack the ribose's C1' and form the product. After dissociation, two further enzymatic reactions convert PreQ1 to queuine (Q), creating queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
Database Links
KEGG: ypy:YPK_3264
Protein Families
Queuine tRNA-ribosyltransferase family
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