Recombinant Yersinia pseudotuberculosis serotype O:3 Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF (arnF)
Description
Molecular Overview
Recombinant Yersinia pseudotuberculosis serotype O:3 ArnF is a 128-amino acid protein (UniProt ID: B1JJ34) expressed in E. coli with an N-terminal His tag . It belongs to the undecaprenyl phosphate-aminoarabinose (L-Ara4N) flippase family, critical for modifying lipid A in bacterial lipopolysaccharides (LPS) to confer resistance to cationic antimicrobial peptides and polymyxins .
Biological Function
ArnF partners with ArnE to form a flippase complex that transports undecaprenyl phosphate-α-L-Ara4N across the inner membrane. This modification of lipid A is essential for:
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Antimicrobial resistance: Reduces LPS net negative charge, limiting cationic peptide binding .
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Virulence regulation: Critical in Yersinia pathogenesis and host immune evasion .
Production Protocols
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Cloning: Full-length arnF gene cloned into E. coli vectors .
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Purification: Affinity chromatography via His-tag, followed by lyophilization .
Research Applications
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Mechanistic studies: Used to dissect lipid A modification pathways .
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Vaccine development: Engineered Yersinia strains expressing modified lipid A (e.g., MPLA) show enhanced immunogenicity in plague vaccine candidates .
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Antimicrobial resistance screening: Target for novel polymyxin adjuvants .
Comparative Analysis with Homologs
| Species | UniProt ID | Length (aa) | Key Differences |
|---|---|---|---|
| Escherichia coli APEC O1 | A1ADB0 | 128 | 78% sequence identity; lower pLDDT (79.85) |
| Salmonella typhi | Q8Z537 | 125 | Truncated C-terminal; 72% identity |
Related Research Findings
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Genetic knockout studies: ΔarnF mutants in Y. pseudotuberculosis exhibit increased susceptibility to polymyxin B .
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Structural insights: Molecular dynamics simulations reveal conformational changes during substrate binding .
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Vaccine adjuvants: Recombinant Y. pseudotuberculosis strains producing MPLA-enriched OMVs induce robust immune responses against Y. pestis .
Product Specs
Please note: We prioritize shipping the format currently in stock. However, if you require a specific format, please specify your preference in the order notes. We will fulfill your request whenever possible.
Please note: All proteins are shipped with standard blue ice packs. If you require dry ice shipping, please contact us in advance. Additional fees may apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. Lyophilized form exhibits a longer shelf life of 12 months at -20°C/-80°C.
Target Background
KEGG: ypy:YPK_1837
