Recombinant Zea mays Aquaporin PIP2-2 (PIP2-2)
Description
Understanding PIP2 Aquaporins in Zea mays
Plasma membrane intrinsic proteins (PIP2) are critical for water and solute transport in plants. In maize (Zea mays), PIP2 isoforms like PIP2;5 and PIP2;6 have been extensively studied for their roles in:
2.1. PIP2;5 in Water Stress and Growth
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Function: Polarized localization in root cells facilitates radial water uptake. Overexpression (OE) enhances leaf elongation under mild stress but not in well-watered conditions .
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Mechanism: Forms heterotetramers with PIP1 isoforms to modulate membrane permeability and pH sensitivity .
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Stomatal Dynamics: Regulates guard cell responses to abscisic acid (ABA), influencing transpiration .
Potential Reasons for the Absence of PIP2-2 Data
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Nomenclature Variability: PIP2 isoforms are often numbered inconsistently across species. For example, Arabidopsis PIP2;5 and rice PIP2;2 have distinct roles .
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Functional Redundancy: Overlapping roles among PIP2 isoforms may reduce the need for targeted studies on PIP2-2.
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Methodological Limitations: Most studies focus on isoforms with clear phenotypes (e.g., PIP2;5 in drought responses) .
4.1. Heteromerization and Regulation
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PIP1–PIP2 Interactions: PIP1 isoforms require PIP2 for plasma membrane localization and enhanced water permeability .
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pH Sensitivity: Coexpression alters gating responses to cytosolic acidification, expanding membrane permeability regulation .
4.2. CO₂ Transport and Photosynthesis
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AtPIP2;5 in Arabidopsis and SiPIP2;7 in sorghum improve mesophyll conductance for CO₂, enhancing photosynthetic efficiency under high vapor pressure deficits .
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Mechanism: CO₂ permeability depends on conserved selectivity filters, suggesting a broad functional potential across PIP2 isoforms .
Recommendations for Future Research
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Clarify Nomenclature: Cross-reference PIP2-2 with maize genomic databases (e.g., MaizeGDB) to confirm its existence.
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Functional Characterization: Use heterologous expression systems (e.g., Xenopus oocytes) to assess PIP2-2’s water/CO₂ permeability and pH gating.
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Comparative Analysis: Study PIP2-2 alongside PIP2;5 and PIP2;6 to identify unique roles or redundancies.
Product Specs
Please note: We prioritize shipping the format currently in stock. However, if you have a specific format requirement, kindly indicate it during order placement, and we will accommodate your request.
Note: Our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please inform us in advance as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
