Recombinant Zygnema circumcarinatum NAD (P)H-quinone oxidoreductase subunit 4L, chloroplastic (ndhE)
Description
Protein Structure and Characteristics
The recombinant ndhE subunit is produced via heterologous expression in Escherichia coli and includes an N-terminal His-tag for purification . Key structural features include:
The protein’s sequence homology with land plant orthologs highlights its evolutionary significance, as Zygnema is a close relative of embryophytes .
Functional Role in Chloroplasts
The ndhE subunit is a component of the NDH complex, which facilitates cyclic electron transport (CET) in chloroplasts. This process generates ATP while minimizing photoinhibition .
Key Functions:
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Cyclic Electron Transport: Contributes to proton gradient formation for ATP synthesis .
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Stress Response: Linked to environmental adaptations in Zygnema, such as desiccation tolerance and low-light survival .
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Evolutionary Adaptation: Orthologs in Zygnema and land plants suggest conserved roles in terrestrialization .
Genomic Context:
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Strain Variability: Z. circumcarinatum strains (e.g., UTEX 1559, SAG 698-1a) show low sequence identity (85.69%) in their plastomes, indicating genomic rearrangements .
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Gene Expansions: Zygnema genomes exhibit expansions in stress-related gene families, including those involved in phytohormone signaling and transmembrane transport .
Applications in Biomedical and Agricultural Research
Challenges and Future Directions
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Strain Authentication: Discrepancies in Z. circumcarinatum strain identities (e.g., SAG 698-1a vs. UTEX 1559) complicate genomic comparisons .
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Functional Elucidation: Limited experimental data on the ndhE subunit’s catalytic role in Zygnema compared to land plants .
Table 2: Genomic Features of Zygnema Strains
Product Specs
Note: We will prioritize shipping the format we have in stock. However, if you have any specific format requirements, please indicate them when placing your order, and we will accommodate your needs.
Note: All protein shipments are standardly accompanied by normal blue ice packs. If you require dry ice shipping, please inform us in advance, as additional charges will apply.
Generally, liquid forms have a shelf life of 6 months at -20°C/-80°C. Lyophilized forms have a shelf life of 12 months at -20°C/-80°C.
