rei-2 Antibody
Description
Applications in Research and Diagnostics
RYR-2 antibodies are widely used in:
Tissue-Specific Expression
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Cardiac Selectivity: Anti-RYR-2 antibodies show strong reactivity in heart tissue lysates but no cross-reactivity in liver or skeletal muscle (Fig. 1A, ).
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Species Reactivity: Confirmed in human, mouse, and rat samples .
Functional Insights
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Calcium Regulation: RYR-2 antibodies help identify dysregulated calcium signaling in cardiac pathologies .
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Autoantibody Implications: While not directly linked to RYR-2, autoantibodies to ACE2 (another cardiac protein) correlate with severe COVID-19 outcomes , highlighting the broader relevance of cardiac autoantibodies.
Validation Data
Western Blot Results:
| Sample | Band Observed | Dilution | Exposure Time |
|---|---|---|---|
| Mouse heart lysate | 564 kDa | 1:1,000 | 15 seconds |
| HEK-293T transfected | 564 kDa | 1:1,000 | 3 seconds |
| Rat liver (negative) | No band | 1:1,000 | 37 seconds |
Clinical and Therapeutic Relevance
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FNAIT Research: While RYR-2 antibodies are not directly used in fetal/neonatal alloimmune thrombocytopenia (FNAIT), monoclonal anti-HPA-1a antibodies (e.g., RLYB212) demonstrate how antibody engineering can prevent alloimmunization .
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Pseudovirus Assays: Neutralization assays for SARS-CoV-2 antibodies provide methodological parallels for quantifying RYR-2 antibody efficacy.
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
