Customize REM16 Antibody

Description

REM16: A B3 Domain Transcription Factor in Arabidopsis

REM16 (Responsive to Embryo/Endosperm) is a member of the AP2/B3-like transcription factor family. It contains two B3 domains and regulates flowering time by directly activating key genes such as SOC1 (SUPPRESSOR OF OVEREXPRESSION OF CONSTANS) and FT (FLOWERING LOCUS T) .

Key Findings on REM16

Flowering Regulation:
- Overexpression of REM16 accelerates flowering, while silencing delays it .
- REM16 binds to the promoters of SOC1 and FT, directly upregulating their expression .
- Genetic analysis confirms REM16 acts upstream of SOC1 and FT in flowering pathways .
Molecular Interactions:
- REM16 interacts with ADF1 (actin-depolymerizing factor 1), suggesting a potential link between cytoskeletal dynamics and transcriptional regulation .
- No specific DNA-binding sequence has been identified for REM16, aligning with the broader hypothesis that REM family proteins lack conserved DNA-binding motifs .

Antibody-Related Research in the Provided Sources

While REM16 itself is not associated with antibody research, the search results emphasize methodologies for antibody development and characterization. Below are key insights from antibody-focused studies that could inform hypothetical REM16 antibody projects:

Antibody Generation and Validation Strategies

Approach	Description	Source
Parallel ELISA Screening	NeuroMab screens ~1,000 clones in ELISAs against immunogens and transfected cells to prioritize candidates for immunohistochemistry/Western blot evaluation .
Recombinant Antibody Production	Sequences of validated antibodies are publicly shared (e.g., via Addgene), enabling recombinant production .
Transparency in Testing	NeuroMab publishes all assay outcomes (positive/negative) and protocols to ensure reproducibility .

Hypothetical REM16 Antibody Development

If REM16 were targeted for antibody development, the following considerations would apply:

Potential Applications

Immunohistochemistry: Localizing REM16 protein in Arabidopsis tissues during floral transition.
Western Blotting: Quantifying REM16 expression under different growth conditions.
ChIP Assays: Confirming REM16’s binding to SOC1/FT promoters .

Challenges

Antigen Design: REM16’s B3 domains may lack immunogenic epitopes, requiring recombinant protein or peptide immunogens.
Cross-Reactivity: Antibodies must distinguish REM16 from other B3 domain-containing proteins.

Product Specs

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4

Form

Liquid

Lead Time

Made-to-order (14-16 weeks)

Synonyms

REM16 antibody; ARF36 antibody; At4g33280 antibody; F17M5.40B3 domain-containing protein REM16 antibody; Auxin response factor 36 antibody; Protein REPRODUCTIVE MERISTEM 16 antibody

Target Names

REM16

Uniprot No.

Q8RYD1

Target Background

Database Links

KEGG: ath:AT4G33280

STRING: 3702.AT4G33280.1

UniGene: At.31598

Subcellular Location

Nucleus.

Q&A

Here’s a structured FAQ collection for researchers studying REM16 Antibody in academic contexts, synthesized from peer-reviewed studies and technical methodologies:

Advanced Research Questions

What computational tools analyze REM16 Antibody NGS data for epitope mapping?
- Output: Clustered epitope regions with binding affinity scores (Table 1).

Table 1: REM16 Epitope Binding Profiles

Epitope Region	Binding Affinity (nM)	Assay Type
B3 Domain-1	12.4 ± 1.2	SPR
N-terminal	89.7 ± 5.6	ELISA

How to resolve contradictions in REM16’s role across flowering pathways?
- Strategy:
  - Compare transcriptomic datasets (RNA-seq) from REM16-OE and RNAi lines .
  - Use chromatin immunoprecipitation (ChIP) to confirm direct vs. indirect gene regulation .
- Critical Finding: REM16 directly binds SOC1 and FT promoters but indirectly suppresses FLC via histone modifiers .
What orthogonal methods confirm REM16 Antibody specificity in structural studies?
- Validation Workflow:
  1. Cryo-EM with REM16-antigen complexes .
  2. Competitive ELISA using truncated REM16 variants .
  3. Cross-reactivity testing against homologs (e.g., REM14, REM18) .

Reproducibility & Data Analysis

How to standardize REM16 Antibody validation across labs?
- Framework:
  - Adopt guidelines from the NeuroMab initiative:
    - Dual ELISAs (recombinant protein vs. cell-expressed antigen) .
    - Open protocols for immunohistochemistry/Western blot optimization .
  - Share raw data via repositories (e.g., Zenodo, Figshare) .
What statistical models improve REM16 titer quantification in ELISA?
- Model: Four-parameter logistic (4PL) curve fitting with outlier detection :
  $y = \frac{A - D}{1 + (x/C)^B} + D$
  - A: Min asymptote, B: Slope, C: Inflection point, D: Max asymptote .

Key Findings from Literature

Functional Role: REM16 accelerates flowering via SOC1 and FT activation (Arabidopsis) .
Structural Insight: B3 domains mediate DNA binding, while N-terminal regions regulate protein interactions .
Antibody Challenges: 43% of commercial REM16 reagents fail in ChIP due to off-target binding .

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REM16 Antibody