REM8 Antibody

FAQs for REM8 Antibody Research
Curated by an experienced research assistant with 15+ years in antibody validation and translational studies

Advanced Research Questions

How to resolve contradictory REM8 localization data between immunofluorescence and Western blot?

• Stepwise approach:

  • Verify fixation/permeabilization protocols match antigen accessibility requirements

  • Compare subcellular fractionation blots with whole-cell lysates

  • Perform proximity ligation assays to confirm spatial relationships

Key consideration: Post-translational modifications may alter epitope availability in different cellular compartments .

What orthogonal methods confirm REM8 antibody target engagement in live cells?

• Combine with:

  • Surface plasmon resonance (KD measurements)

  • Native SEC-MS for complex stability analysis

  • Phage display mutagenesis to map epitope-paratope interactions

Data correlation matrix:

How to design longitudinal studies tracking REM8 antibody pharmacokinetics?

• Critical parameters:

  • Sample collection timing aligned with predicted IgG half-life (21-28 days)

  • Dual detection systems (e.g., anti-idiotype ELISA + neutralizing assays)

  • Baseline normalization against pre-immunization samples

Methodological Challenges

Standardizing REM8 antibody lot-to-lot variability in multicenter trials

• Implement:

  • ISO/IEC 17025 compliant testing for binding kinetics

  • Accelerated stability studies (40°C/75% RH for 3 months equivalent to 2 years)

  • Consensus scoring of histopathology slides by ≥3 blinded pathologists

Analyzing REM8 cross-reactivity with homologous proteins

• Recommended workflow:

  • Phylogenetic analysis of target protein family

  • Peptide microarray screening against all human paralogs

  • Molecular dynamics simulations of antibody-antigen complexes