At2g24690 Antibody

Functional Characterization

AT2G24690 belongs to the REM gene family, which regulates reproductive meristem development. Experimental studies highlight its role in:

• Phyllotaxis and Inflorescence Development: REM proteins, including those encoded by AT2G24690, interact with auxin response factors (e.g., ARF19) to modulate auxin signaling pathways critical for organ positioning .

• Genetic Interactions: AT2G24690 forms protein complexes with REM34 and REM35, which suppress auxin-responsive genes like LBD18 in shoot apical meristems .

• Mutant Phenotypes: Knockout mutants exhibit defects in floral organ patterning and phyllotactic irregularities, underscoring its role in maintaining meristematic integrity .

Research Applications

The At2g24690 antibody has been utilized in:

• Immunolocalization: Tracking protein expression in Arabidopsis tissues, particularly in meristematic regions .

• Protein-Protein Interaction Studies: Co-immunoprecipitation assays to identify binding partners (e.g., ARF19) .

• Gene Expression Analysis: Validating transcriptional repression mechanisms in auxin signaling pathways .

Key Research Findings

Recent studies employing the At2g24690 antibody have revealed:

1. Auxin Signaling Modulation: REM proteins sequester ARF19, limiting its availability to activate auxin-responsive genes .

2. Developmental Plasticity: AT2G24690 ensures precise phyllotactic patterning by dampening auxin response variability in meristems .

3. Evolutionary Conservation: REM family members share structural motifs with reproductive regulators in other plant species .

Limitations and Future Directions

While the antibody is validated for basic research, its utility in non-model plants remains untested. Future work could explore:

• Tissue-specific expression profiles using CRISPR-edited reporter lines.

• Cross-species reactivity in Brassicaceae relatives.

This antibody remains indispensable for dissecting the molecular basis of plant development, with implications for crop engineering and developmental biology.