UPF3 Antibody
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In Stock 
Product Specs
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
UPF3 antibody; At1g33980 antibody; F12G12.20 antibody; T15K4.3 antibody; Regulator of nonsense transcripts UPF3 antibody; Nonsense mRNA reducing factor UPF3 antibody; Up-frameshift suppressor 3 homolog antibody; AtUpf3 antibody
Target Names
UPF3
Uniprot No.
Target Background
Function
This antibody targets UPF3, a protein crucial for the nonsense-mediated decay (NMD) pathway. UPF3 plays a key role in recognizing and degrading mRNAs containing premature termination codons (PTCs), preventing the production of truncated and potentially harmful proteins. It acts by forming a complex with UPF1 and UPF2 at the cytoplasmic side of the nuclear envelope, where it binds to spliced mRNA upstream of exon-exon junctions. This complex, along with UPF1 bound to release factors at the stalled ribosome, activates NMD, ensuring proper mRNA surveillance and quality control. UPF3 is essential for plant development and adaptation to environmental stresses, including defense against pathogens and response to wounding. It effectively eliminates the production of nonsense-containing RNAs (ncRNAs).
Gene References Into Functions
- Research indicates that, unlike mammalian cells, maintaining a precise balance of UPF3 transcript levels through feedback loops and transcription regulation is critical for accurate NMD regulation in Arabidopsis. PMID: 25820429
- A comprehensive analysis of splice variants in an Arabidopsis mutant lacking UPF3, a core component of the NMD machinery, revealed the degradation of PTC-containing transcripts by NMD. PMID: 19863731
- The AtUPF3 gene is essential for the plant nonsense-mediated mRNA decay system, effectively preventing the accumulation of aberrantly spliced mRNA. PMID: 16098107
- Studies demonstrate that in mutants with impaired NMD components, specifically UPF3, levels of the alternatively spliced AtGRP7 and AtGRP8 transcripts are significantly elevated, highlighting the role of UPF3 in the decay of these clock-regulated transcripts. PMID: 17924945
- The localization of UPF3 to the nucleolus suggests that this organelle is involved in identifying aberrant mRNAs and initiating the NMD pathway in Arabidopsis. PMID: 19602621
Database Links
Protein Families
RENT3 family
Subcellular Location
Nucleus, nucleolus. Nucleus. Cytoplasm.
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