REV1 Antibody

Preservative: 0.03% ProClin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Liquid

14-16 weeks (Made-to-order)

REV1 antibody; At5g44750 antibody; K23L20.9 antibody; T19K24.14 antibody; DNA repair protein REV1 antibody; AtREV1 antibody; EC 2.7.7.- antibody; Rev1-like terminal deoxycytidyl transferase antibody

REV1

A3EWL3

REV1 is a deoxycytidyl transferase crucial for DNA repair and translesion synthesis (TLS). It catalyzes the template-dependent addition of a dCMP residue from dCTP to the 3'-end of a DNA primer. Furthermore, REV1 mediates the insertion of dTMP or dGMP opposite a guanine base, and less efficiently, inserts dGMP opposite G, T, and C; dAMP opposite G, A, and T; and dTMP opposite A. This enzyme likely plays a critical role in the initial bypass of abasic DNA lesions by inserting a nucleotide opposite the lesion. REV1 is essential for the normal induction of mutations caused by physical and chemical agents (e.g., UV and gamma radiation), primarily through G to T transversions, and spontaneous mutations in somatic cells. Its function confers resistance to ultraviolet-B (UV-B) radiation and various DNA cross-linking agents (e.g., mitomycin C and cisplatin). Additionally, REV1 promotes stem growth.

The role of REV1 has been extensively studied: 1. A translesion synthesis mechanism in higher plants has been identified, with AtREV1 and AtREV7 playing significant roles in tolerating DNA-damaging agents. (PMID: 15908599) 2. In vitro primer extension assays have been utilized to elucidate the functional mechanisms of REV1. (PMID: 17827267) 3. Transgenic plants with either overexpressed or disrupted REV1 exhibited reduced germination rates; however, plants with REV1 overexpression demonstrated enhanced stem growth compared to wild-type plants. (PMID: 18339443)

KEGG: ath:AT5G44750

STRING: 3702.AT5G44750.2

UniGene: At.30056

DNA polymerase type-Y family

Nucleus.