rgpB Antibody
Description
Structure and Function of RgpB
RgpB is a thiol protease that works synergistically with RgpA to degrade host proteins, facilitating immune evasion and tissue destruction in periodontitis . Its Ig-like domain is a critical epitope for antibody recognition . The protein’s proteolytic activity enables P. gingivalis to modify host immune molecules, evade defense mechanisms, and induce bone resorption .
Applications in Research
The rgpB Antibody is primarily used in:
Rheumatoid Arthritis (RA)
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Elevated anti-RgpB IgG levels correlate with RA diagnosis (OR=2.96; CI: 2.00–4.37) .
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Saliva IgA anti-RgpB antibodies associate with RA disease activity (Spearman’s ρ=0.32, p<0.05) .
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Longitudinal studies suggest anti-RgpB antibodies precede RA symptoms by ~5 years .
Periodontitis
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RgpB activity in P. gingivalis drives periodontal tissue destruction .
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No direct association between anti-RgpB antibodies and periodontitis severity .
Alzheimer’s Disease
Diverse Research Sources
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
The rgpB polyclonal antibody is generated by immunizing rabbits with the recombinant Porphyromonas gingivalis Gingipain R2 protein (amino acids 230-473), serving as the immunogen. This immunization process elicits the production of antibodies specifically targeting the Porphyromonas gingivalis rgpB antigen. Subsequently, these antibodies are purified from the rabbit serum through a protein G purification method, achieving a purity level exceeding 95%. The resulting purified rabbit anti-Porphyromonas gingivalis rgpB polyclonal antibody exhibits high specificity for rgpB and demonstrates a strong affinity for the antigen. This rgpB antibody is suitable for use in ELISA assays.
The rgpB protein primarily functions in the degradation and modification of host proteins, particularly those involved in the immune response, facilitating the survival of P. gingivalis within the host. Additionally, it plays a significant role in regulating gene expression within P. gingivalis and inducing tissue destruction and bone resorption associated with periodontitis.
Target Background
KEGG: pgi:PG_0506
STRING: 242619.PG0506
Customer Reviews
Applications : western blot
Sample type: cells
Review: Rgp-specific and Kgp-specific proteolytic activities were determined in whole P. gingivalis cultures grown for 24 h in iron- and heme-rich conditions (Hm) or in iron_x005fand heme-free conditions (DIP).
