rhp26 Antibody
Description
Functional Insights from Rhp26 Antibody Studies
Rhp26 antibodies have elucidated its bidirectional regulatory mechanisms:
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Chromatin remodeling: Rhp26 disrupts histone cores (H3/H4) and releases free DNA, dependent on H4 tails .
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DNA repair: Rhp26 collaborates with Ddb1-Cul4 ubiquitin ligase in TC-NER. Antibody-based assays show Rhp26 ubiquitination post-UV irradiation .
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Transcription regulation: Rhp26 resolves RNA polymerase II (RNAPII) pausing at R-loop-prone regions, preventing transcription-replication conflicts .
Mechanistic Comparison with Orthologs
| Protein | Organism | Key Function | Dependency on Histone Tails |
|---|---|---|---|
| Rhp26 | S. pombe | Nucleosome eviction, TC-NER | H4 > H3 |
| Rad26 | S. cerevisiae | RNAPII backtracking resolution | N/A |
| CSB | Humans | Transcription-coupled repair | H3/H4 |
Data derived from chromatin-remodeling assays .
Chromatin-Remodeling Activity
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Nucleosome disruption: Rhp26 mutants (e.g., Rhp26 7P/10P) show enhanced nucleosome eviction, confirmed via electrophoretic mobility shift assays .
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Histone tail dependence: Rhp26 activity decreases by 60% on tailless (TL) nucleosomes compared to wild type (WT) .
DNA Damage Response
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UV resistance: Rhp26-deficient strains exhibit hypersensitivity to UV, rescued by functional Rhp26 expression .
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Epistasis analysis: Rhp26 operates in a pathway distinct from global genome repair (GGR) proteins like Rhp23/XPC .
Genomic Localization (HOOD-29 Region)
| Genomic Region | Coordinates (Chr3) | Associated Features | Rhp26 Binding |
|---|---|---|---|
| HOOD-29 | 1,179,500–1,182,650 | rhp26 gene, H3K9me2 modification | + |
Data from genome-wide ChIP-seq .
Applications of Rhp26 Antibodies
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Ubiquitination assays: Detect post-translational modifications of Rhp26 under DNA damage .
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Chromatin immunoprecipitation (ChIP): Map Rhp26 binding sites at R-loop-prone genomic loci .
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Functional studies: Validate Rhp26 mutants in S. pombe survival assays .
Challenges and Limitations
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- The leucine latch motif, present in organisms ranging from yeast to humans, suggests a conserved mechanism for the autoregulation of CSB/ERCC6. This mechanism is conserved despite the highly divergent nature of the N- and C-terminal regions. PMID: 25512493
- Fission yeast homologs of human XPC and CSB, rhp41 and rhp26, respectively, are involved in transcription-coupled repair of methyl methanesulfonate-induced DNA damage. PMID: 21670547
KEGG: spo:SPCP25A2.02c
STRING: 4896.SPCP25A2.02c.1
