RHPN1 Antibody
Description
Introduction to RHPN1 and Its Antibody
RHPN1 (Rhophilin Rho GTPase Binding Protein 1) is a protein-coding gene involved in signal transduction, focal adhesion assembly, and cytoskeletal regulation . It interacts with Rho GTPases, which are critical for cellular processes like motility and division. The RHPN1 antibody targets this protein, enabling researchers to investigate its expression, localization, and functional roles in both normal and pathological states.
Recommended Dilutions
| Application | Dilution Range |
|---|---|
| Western Blot (WB) | 1:1,000 – 1:8,000 |
| Immunoprecipitation (IP) | 0.5–4.0 µg per 1–3 mg of lysate |
This antibody has been tested in diverse samples, including human brain tissue, A375 melanoma cells, and Jurkat T-cells .
Research Applications
The RHPN1 antibody is utilized to:
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Study Rho GTPase Signaling Pathways: RHPN1 is a Rho-binding protein implicated in cytoskeletal dynamics and cell adhesion .
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Investigate Disease Mechanisms: Dysregulation of RHPN1 is linked to cancers (e.g., ovarian, glioma) and genetic disorders like Blepharocheilodontic Syndrome 1 .
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Validate Protein Expression: Used in Western blotting to confirm RHPN1 levels in cell lines (e.g., HEY ovarian cancer cells) and tissues .
Key Research Findings
While the RHPN1 antibody itself is not directly discussed in mechanistic studies, its target protein, RHPN1, is implicated in oncogenic processes:
Role in Cancer
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Ovarian Cancer: RHPN1’s antisense RNA (RHPN1-AS1) promotes tumor progression by sponging miRNAs (e.g., miR-485-5p, miR-6884-5p) and upregulating oncogenes like TPX2 and TOP2A . Although these studies focus on the RNA, the antibody could help correlate RHPN1 protein levels with RNA-driven phenotypes.
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Head and Neck Squamous Cell Carcinoma (HNSCC): RHPN1-AS1 overexpression enhances cell migration and invasion via epithelial-mesenchymal transition (EMT) .
Cellular Functions
Limitations and Future Directions
Current research on RHPN1 primarily focuses on its antisense RNA rather than the protein. Further studies using the RHPN1 antibody could clarify:
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Protein-RNA interactions in carcinogenesis.
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Subcellular localization of RHPN1 in disease models.
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Therapeutic targeting of RHPN1 in Rho-dependent pathologies.
