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Description

Introduction to RIC1 Antibody

RIC1 antibodies are immunological reagents designed to detect RIC1 homolog (RAB6A GEF complex partner 1), a 159.3 kDa cytoplasmic protein involved in vesicular trafficking. These antibodies target epitopes within specific regions of the 1423-amino acid protein (e.g., residues 950-1250 in ab234741) .

Key Applications

RIC1 antibodies are widely used in:

Western Blot: Detecting endogenous RIC1 protein levels
Immunohistochemistry (IHC-P): Localizing RIC1 in tissue sections
Immunofluorescence (ICC/IF): Visualizing Golgi-associated RIC1-RGP1 complexes
ELISA: Quantifying RIC1 expression under experimental conditions

Functional Insights from Research

The RIC1-RGP1 complex exhibits guanine nucleotide exchange factor (GEF) activity critical for cellular processes:

Process	Mechanism	Experimental Evidence
Rab6A activation	GDP-to-GTP exchange on Rab6A	Required for endosome-Golgi vesicle fusion
Collagen transport	Regulates procollagen II secretion via TGN	Zebrafish mutants show intracellular collagen retention
Craniofacial development	Maintains cartilage morphogenesis	ric1−/− zebrafish exhibit microcephaly and malformed cartilage

Studies demonstrate that RIC1 depletion destabilizes Rab6, impairing retrograde transport of mannose-6-phosphate receptors (MPRs) .

Disease Associations

RIC1 dysfunction is implicated in:

Condition	Pathogenic Mechanism	Clinical Correlation
CATIFA syndrome	Homozygous RIC1 mutations	Cleft lip, cataracts, ADHD, skeletal defects
Connective tissue disorders	Impaired collagen secretion	Zebrafish models show tendon/muscle attachment defects

BioVU biobank analysis links reduced RIC1 expression to musculoskeletal, respiratory, and neurodevelopmental phenotypes (OR: 1.3–2.1 for associated traits) .

Research Validation

Key findings using RIC1 antibodies include:

Rab33B-GTP binding at Ric1's C-terminal domain mediates Rab6 activation cascades
RIC1 knockdown reduces Rab6-GTP levels by 67±8% (p<0.01)
Patient-derived fibroblasts show 50% reduced RIC1 transcript levels

Product Specs

Buffer

Preservative: 0.03% ProClin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Lead Time

14-16 weeks (Made-to-order)

Synonyms

RIC1 antibody; At2g33460 antibody; F4P9.23 antibody; CRIB domain-containing protein RIC1 antibody; ROP-interactive CRIB motif-containing protein 1 antibody; Target of ROP protein RIC1 antibody

Target Names

RIC1

Uniprot No.

F4IVV0

Target Background

Function

RIC1 functions as a downstream effector of Rho-related GTPases from the Plant ROP (ROP) family. It plays a crucial role in propagating ROP GTPase signals, mediating specific cellular responses. RIC1 is essential for cortical microtubule organization, promoting microtubule bundling and the formation of ordered arrays, particularly in the neck region of pavement cells. This process restricts lateral cell expansion, contributing to the characteristic narrow neck morphology of these cells. RIC1's function is inhibited by interaction with activated ARAC4/ROP2. Conversely, RIC1 represses ARAC4/ROP2 activation and antagonizes the RIC4-actin pathway, which promotes cortical actin microfilament assembly. RIC1 also acts as a downstream effector of ARAC3/ROP6, participating in a signaling pathway that negatively regulates clathrin-mediated endocytosis and the internalization of PIN1 and PIN2 auxin transporters. This involvement is critical for asymmetric auxin distribution during root gravitropism and vascular patterning. RIC1 positively regulates auxin responses but negatively regulates abscisic acid (ABA) responses during lateral root development and primary root elongation.

Gene References Into Functions

RIC1's Role in Plant Development and Stress Response: Evidence from Published Studies

The ROP2-RIC1 pathway finely tunes microtubule dynamics in response to salt stress in Arabidopsis. ROP2 enhances salt tolerance by suppressing RIC1. PMID: 28070891
RIC1 knockout mutants exhibit a lack of cellulase-induced inhibition of cell interdigitation, indicating its role in this process. PMID: 28011873
RIC1 regulates F-actin dynamics at the apical plasma membrane and in the cytosol by severing F-actin and capping barbed ends, influencing pollen tube growth. PMID: 25804540
RIC1 knockout suppresses the effects of auxin on lateral root formation and primary root elongation. PMID: 23078108
The ROP6-RIC1 pathway links cytoplasmic auxin signaling to the regulation of cortical microtubule organization and cell elongation. PMID: 23394835
SPIKE1 (SPK1) mutations induce PIN2 internalization, an effect not suppressed by auxin or by loss-of-function mutations in ROP6 or RIC1. SPK1 is required for auxin-induced ROP6 activation. PMID: 22683260
ROP6 and RIC1 regulate clathrin association with the plasma membrane for clathrin-mediated endocytosis and its feedback regulation by auxin. Genetic analyses indicate ROP6/RIC1 acts downstream of ABP1 to regulate endocytosis. PMID: 22683261

Database Links

KEGG: ath:AT2G33460

STRING: 3702.AT2G33460.1

UniGene: At.37923

Subcellular Location

Cytoplasm, cytoskeleton. Cytoplasm.

Tissue Specificity

Expressed in columella cells from the root tip and epidermal cells at the base of lateral roots, leaves, stems, flowers, anthers, pollen and siliques.

Customer Reviews

Overall Rating 5.0 Out Of 5

,

B.A

By Anonymous

★★★★★

Applications : Immunohistochemistry

Sample type: serum

Review: IHC staining showing AAV-Runx1 overexpression-inducedchanges in RIC1 expression in articular cartilage at 12 weeks after ACLT surgery.

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RIC1 Antibody