RIT2 Antibody
Description
Introduction to RIT2 Antibody
RIT2 (Ras-like in neurons) antibodies are specialized research tools designed to detect and study the RIT2 protein, a small GTPase critical for neuronal signaling, autophagy regulation, and Parkinson’s disease (PD) pathology . These antibodies enable precise visualization and quantification of RIT2 in biochemical assays, immunohistochemistry (IHC), and molecular studies. RIT2 is enriched in dopaminergic neurons of the substantia nigra (SNc) and interacts with LRRK2 kinase, α-synuclein (aSyn), and autophagy-lysosomal pathway (ALP) components .
Applications in Research
RIT2 antibodies are employed in diverse experimental workflows:
Western Blotting (WB)
Immunohistochemistry (IHC)
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Localizes RIT2 in mouse pancreas and brain tissue (e.g., SNc dopaminergic neurons) .
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Requires antigen retrieval (TE buffer pH 9.0 or citrate buffer pH 6.0) .
Immunoprecipitation (IP)
ELISA
Research Findings Linking RIT2 Antibodies to Neurological Pathways
RIT2 antibodies have elucidated critical roles of RIT2 in neurodegeneration:
Parkinson’s Disease (PD) Pathology
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LRRK2 Kinase Modulation: RIT2 overexpression reduces LRRK2 kinase activity (pS1292-LRRK2) and rescues autophagy-lysosomal deficits in G2019S-LRRK2 cells .
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α-Synuclein (aSyn) Aggregation: RIT2 overexpression diminishes pS129-aSyn inclusions and protects against A53T-aSyn-induced neurodegeneration in vivo .
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Lysosomal Function: RIT2 enhances lysosomal biogenesis, reduces lysosome size, and increases proteolytic activity (DQ-Red-BSA assay) .
Autophagy-Lysosomal Pathway (ALP) Regulation
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RIT2 upregulates MiT/TFE3 transcription factors, promoting ALP gene expression (e.g., lysosomal hydrolases) .
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RIT2 deficiency mimics ALP defects seen in PD models, highlighting its role in proteostasis .
Clinical Relevance
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
RIT2 Gene and its Association with Neurological Disorders
- RIT2 has emerged as a potential susceptibility gene for neurological disorders, affecting millions worldwide. Studies have identified various genetic variations, including single nucleotide polymorphisms (SNPs) and copy number variations (CNVs), in different populations. PMID: 29860660
- Associations between RIT2 polymorphisms and Parkinson's disease and essential tremor have been observed, specifically with SNP rs12456492. PMID: 26941103
- A RIT2 gene variant has been identified as a risk factor for autism spectrum disorder in Iranian patients. PMID: 28190241
- Research suggests a selective advantage for a human-specific allele at a short tandem repeat locus within the human RIT2 core promoter. PMID: 28214997
- RIT2 rs12456492 has not been shown to increase the risk of Parkinson's disease in Han Chinese populations. PMID: 27889863
- Meta-analysis indicates a possible association between rs12456492 and increased Parkinson's disease risk in Asian populations. However, further studies with larger sample sizes and diverse ethnic groups are needed to confirm this association. PMID: 26334395
- Findings strengthen the likelihood of a connection between Parkinson's disease and RIT2 variation in Asian populations. PMID: 25146456
- A case-control replication study in a large cohort of Chinese Han individuals investigated the RIT2 rs12456492 variant. PMID: 25559334
- Polymorphism in the RIT2 gene has been linked to Parkinson's disease in a Han Chinese population. PMID: 25457028
- Data suggests an association between RIT2 polymorphisms and the etiology of Parkinson's disease. PMID: 25534083
- Meta-analysis suggests that the G allele and GG and GA genotypes of rs12456492(A/G) polymorphism might increase the risk of Parkinson's disease. PMID: 26188085
- Studies have failed to replicate the RIT2 rs12456492 variant as a genetic risk factor for Parkinson's disease in certain populations. PMID: 23635658
- Research confirms the expression of RIT2 in retinal neuronal cells. PMID: 23805044
- Meta-analysis identified RIT2 as a novel Parkinson's disease locus, replicating several previously identified loci on chromosome 18. PMID: 22451204
- Rin (RIT2) interacts directly with the dopamine transporter (DAT) in a protein kinase (PK)C-regulated manner and is essential for PKC-mediated DAT internalization. PMID: 21957239
- RIT2 modulates the activation of the Brn-3a regulated egr-1 promoter by the N-terminal domain of Brn-3a. PMID: 12934100
- The neuron-specific small GTPase Rin (RIT2) is involved in downstream signaling of plexin B3. PMID: 16122393
- Findings establish RIT2 as a neuronal-specific regulator of neurotrophin signaling, required to couple NGF stimulation to sustain activation of p38 MAP kinase and b-Raf signaling cascades essential for neuronal development. PMID: 16157584
