| Parameter | Details |
|---|
| Host species | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptides (e.g., residues 71–100 , 1–150 , or 61–110 ) |
| Dilution range (WB) | 1:500–1:2000 ; 1:1000 |
| Storage | -20°C in PBS with 0.02% sodium azide and 50% glycerol |
Tested Applications
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Western Blot (WB): Detected in A549, HeLa, Jurkat, and NIH3T3 cell lines .
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Immunohistochemistry (IHC): Validated in human placenta, endometrial cancer, and mouse/rat brain/stomach tissues .
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ELISA: Compatible with human and mouse samples .
Performance Data
| Application | Recommended Dilution | Key Findings |
|---|
| WB | 1:500–1:2000 | Band observed at ~21 kDa in human, mouse, and rat lysates . |
| IHC | 1:500–1:2000 | Strong cytoplasmic staining in cancer tissues and normal brain/stomach . |
Role in Ribophagy
A 2025 study identified RPL12 as a conserved ribophagy receptor essential for cellular homeostasis :
Disease Associations