RPL12C Antibody

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Description

ParameterDetails
Host speciesRabbit
ClonalityPolyclonal
ImmunogenSynthetic peptides (e.g., residues 71–100 , 1–150 , or 61–110 )
Dilution range (WB)1:500–1:2000 ; 1:1000
Storage-20°C in PBS with 0.02% sodium azide and 50% glycerol

Tested Applications

  • Western Blot (WB): Detected in A549, HeLa, Jurkat, and NIH3T3 cell lines .

  • Immunohistochemistry (IHC): Validated in human placenta, endometrial cancer, and mouse/rat brain/stomach tissues .

  • ELISA: Compatible with human and mouse samples .

Performance Data

ApplicationRecommended DilutionKey Findings
WB1:500–1:2000Band observed at ~21 kDa in human, mouse, and rat lysates .
IHC1:500–1:2000Strong cytoplasmic staining in cancer tissues and normal brain/stomach .

Role in Ribophagy

A 2025 study identified RPL12 as a conserved ribophagy receptor essential for cellular homeostasis :

  • Mechanism: RPL12 binds autophagy proteins Atg8 and Atg11 during starvation, promoting ribosome degradation.

  • Functional impact:

    • RPL12 deficiency accelerates cell death under nutrient stress.

    • Overexpression improves motor defects in Drosophila models of aging and amyloid-β toxicity .

Disease Associations

  • Cancer: Elevated RPL12 expression in endometrial and gastric carcinomas .

  • Neurodegeneration: Linked to aging-related motor deficits and protein aggregation .

Product Specs

Buffer
Preservative: 0.03% ProClin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
14-16 week lead time (made-to-order)
Synonyms
RPL12C antibody; At3g27850 antibody; K16N12.7 antibody; K16N2.21 antibody; 50S ribosomal protein L12-3 antibody; chloroplastic antibody; CL12-C antibody
Target Names
RPL12C
Uniprot No.

Target Background

Database Links

KEGG: ath:AT3G27850

STRING: 3702.AT3G27850.1

UniGene: At.20381

Protein Families
Bacterial ribosomal protein bL12 family
Subcellular Location
Plastid, chloroplast.

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