RPL4A Antibody

Introduction to RPL4A Antibody

RPL4A antibodies are polyclonal or monoclonal reagents designed to detect the RPL4A protein across species, including humans, mice, rats, and plants. These antibodies are widely used in techniques such as Western blot (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), and immunofluorescence (IF). Their applications span molecular biology, cancer research, and studies of ribosomal biogenesis .

General Properties

Validation Data

• Western Blot: Detects a single band at 48 kDa in human HeLa, 293T, and mouse pancreas lysates .

• IHC: Strong cytoplasmic staining in human colon cancer, mouse bladder, and rat tissues .

• IF: Nuclear and cytoplasmic localization in U2OS cells .

Role in Ribosomal Biogenesis

• RPL4A requires dedicated chaperones like Acl4 for co-translational folding and nuclear import in yeast. Mutations in RPL4A disrupt ribosome assembly and impair growth .

• In Arabidopsis, RPL4A regulates auxin-mediated ribosomal biogenesis, linking protein synthesis to vacuolar trafficking .

Regulatory Mechanisms

• mRNA Stability: RPL4A mRNA levels are tightly controlled. Disruption of chaperones (e.g., Caf130, EGD2) increases mRNA stability, suggesting a feedback loop between protein synthesis and transcript regulation .

• Compensatory Expression: In Arabidopsis, loss of RPL4A upregulates its paralog RPL4D, highlighting functional redundancy .

Disease Associations

• Aberrant RPL4 expression correlates with developmental defects and cancer progression. Antibodies have identified RPL4 overexpression in colorectal and pancreatic tissues .

Applications of RPL4A Antibodies

• Storage: Maintain at -20°C in glycerol-containing buffers to prevent freeze-thaw damage .

• Controls: Include lysates from RPL4A-knockdown cells (e.g., Arabidopsis rpl4a mutants) to confirm specificity .

• Cross-Reactivity: Most antibodies recognize both RPL4A and its paralogs (e.g., RPL4D in plants) .