RMDN3 Antibody, FITC conjugated
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Description
Key Applications
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IHC/IF: Detects RMDN3 in paraffin-embedded tissues (e.g., liver cancer, lung adenocarcinoma) and cell lines (U2OS) .
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Flow Cytometry: Used to study RMDN3-VAPB interactions in mitochondrial ROS-induced ER-mitochondria contact sites (MERCs) .
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Western Blot: Validates FITC conjugation efficiency and antibody specificity .
Research Findings
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Role in MERCs: RMDN3 phosphorylation at T160 enhances binding to VAPB, promoting MERCs formation under mitochondrial oxidative stress. This facilitates lipid radical transfer to the ER, reducing mitochondrial toxicity .
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Calcium Regulation: RMDN3 knockdown reduces mitochondrial calcium uptake during rotenone/antimycin A treatment .
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Apoptosis: Overexpression induces apoptosis in keratinocytes, suggesting therapeutic relevance .
Conjugation Methodology
FITC conjugation involves:
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Antibody Preparation: Dialysis into reaction buffer (pH 9.5) to remove sodium azide .
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FITC Reaction: Incubation with FITC (40–80 µg per mg antibody) in DMSO for 1 hour .
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Quality Control:
Validation Data
Limitations and Considerations
Product Specs
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Typically, we can ship your order within 1-3 business days of receiving it. Delivery times may vary depending on your location and shipping method. Please consult your local distributor for specific delivery timeframes.
Synonyms
RMDN3 antibody; FAM82A2 antibody; FAM82C antibody; PTPIP51 antibody; hucep-10 antibody; UNQ3122/PRO10274 antibody; Regulator of microtubule dynamics protein 3 antibody; RMD-3 antibody; hRMD-3 antibody; Cerebral protein 10 antibody; Protein FAM82A2 antibody; Protein FAM82C antibody; Protein tyrosine phosphatase-interacting protein 51 antibody; TCPTP-interacting protein 51 antibody
Target Names
RMDN3
Uniprot No.
Target Background
Function
PTPIP51 plays a critical role in regulating cellular calcium homeostasis. It may also be involved in keratinocyte differentiation and apoptosis. Overexpression of PTPIP51 has been shown to induce apoptosis.
Gene References Into Functions
- Research suggests that the VAPB-PTPIP51 tethers regulate autophagy. Overexpression of VAPB or PTPIP51, which strengthens endoplasmic reticulum-mitochondria contacts, impairs autophagosome formation. Conversely, silencing VAPB or PTPIP51, which loosens these contacts, stimulates autophagosome formation. PMID: 28132811
- This review summarizes recent findings concerning the function, regulation, and protein complex formation of PTPIP51, with a focus on the involved signaling pathways and subcellular compartments. PMID: 27734150
- High PTPIP51 expression has been correlated with glioblastoma. PMID: 25862004
- PTPIP51's activity is regulated by its phosphorylation status, which also influences its subcellular localization. PMID: 24501773
- Studies have demonstrated that PTPIP51 interacts with 14-3-3beta and Raf-1 in vivo in a tyrosine phosphorylation-dependent manner. Additionally, it interacts with DAGKalpha and PKA in a tyrosine-dependent manner. PMID: 22544307
- In glioblastoma, PTPIP51 expression increases with the grade of malignancy, and it interacts with 14-3-3ss and PTP1B. PMID: 21972092
- PTPIP51 is phosphorylated by Lyn and c-Src kinases, but not dephosphorylated by PTP1B in acute myeloid leukemia. PMID: 21513978
- PTPIP51 expression is restricted to myeloid precursor cells undergoing differentiation. In blood cells, PTPIP51 expression is specifically observed in differentiating and mature neutrophil granulocytes. PMID: 20627780
- PTPIP51 may be involved in regulating cellular processes associated with differentiation, movement, or cytoskeletal organization. PMID: 15609043
- PTPIP51 is a mitochondrial protein with apoptosis-inducing function, and its N-terminal transmembrane domain is essential for both its mitochondrial targeting and its apoptotic functions. PMID: 16820967
- PTPIP51 and PTP1B play roles in the differentiation and apoptosis of the cytotrophoblast and syncytiotrophoblast, respectively. Additionally, PTPIP51 may serve as a signaling partner in angiogenesis and vascular remodeling. PMID: 18854601
- FAM82A2 is expressed in keratinocyte carcinoma and their surrounding stroma. PMID: 19012732
- Data strongly suggest a strong association between PTPIP51 expression and differentiation in human muscle cells. PMID: 19124842
- The methylation status of the PTPIP51 promoter appears to influence its expression, which is elevated in prostate carcinoma. PMID: 19691131
Database Links
Protein Families
RMDN family
Subcellular Location
Mitochondrion outer membrane; Single-pass membrane protein. Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole.
Tissue Specificity
Present at high level in epidermis and seminiferous epithelium: while basal cells in the epidermis and spermatogonia show no perceptible amount, keratinocytes of suprabasal layers and differentiating first-order spermatocytes up to spermatids exhibit high
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