RNF113B Antibody

What is RNF113B Antibody?

RNF113B antibodies are immunological reagents designed to bind specifically to the RNF113B protein, encoded by the RNF113B gene (UniProt: Q8IZP6, KEGG: hsa:140432) . These antibodies enable researchers to investigate the protein’s expression, localization, and function in human tissues, particularly in cancer and DNA damage response pathways.

Validation and Specificity

RNF113B antibodies are rigorously validated:

• Western Blot: Detects endogenous RNF113B at ~37 kDa . Example: Boster Bio’s antibody (A18242) shows reactivity in COLO205 cell lysates .

• Immunoprecipitation: Sigma-Aldrich’s monoclonal antibody (SAB1403462) confirms specificity via IP .

• Cross-Reactivity: No observed cross-reactivity with unrelated proteins .

4.1. Cancer Biology

• RNF113B is implicated in lung adenocarcinoma, with elevated expression observed in tumor tissues compared to normal lung parenchyma .

• Antibodies enable studies on RNF113B’s role in DNA damage response (DDR) and resistance to chemotherapeutics like Cisplatin .

4.2. Functional Studies

• CRISPR/Cas9 knockout plasmids (e.g., Santa Cruz Biotechnology’s sc-414479) paired with RNF113B antibodies help dissect gene function in vitro .

• RNF113B deficiency correlates with increased R-loop formation and spliceosome destabilization, linking it to genomic instability .

Technical Considerations

• Dilutions: Optimal working dilutions vary:

  • ELISA: 1:10,000

  • WB: 1:500–1:3,000

• Blocking Peptides: Available for competitive assays (e.g., Boster Bio’s custom service) .