RNF150 Antibody

Definition and Purpose of RNF150 Antibody

The RNF150 antibody is a polyclonal immunoglobulin targeting the C-terminal region of the RING finger protein 150 (RNF150), a member of the E3 ubiquitin ligase family. It is primarily used to detect RNF150 in experimental workflows such as Western blotting (WB) and immunohistochemistry (IHC) . Its immunogen is a synthetic peptide corresponding to residues within the C-terminal sequence of human RNF150 .

Research Applications in Gastric Cancer

The RNF150 antibody has been instrumental in elucidating RNF150’s role in GC pathogenesis:

Protein Interactions and Functional Insights

PPI network analysis identified interactions between RNF150 and ubiquitin-conjugating enzymes (e.g., UBE2U, UBE2Z) , supporting its role in protein ubiquitination. Additionally, RNF150 showed strong correlations with genes like RSPO3 (r = 0.8239) , suggesting involvement in Wnt signaling modulation.

Challenges and Future Directions

• Technical Limitations: Current studies rely heavily on mRNA and IHC data; further validation via Western blotting and immunofluorescence is needed .

• Functional Studies: The exact mechanism of RNF150 in GC remains unclear, necessitating in vitro and in vivo experiments .