anti-Homo sapiens (Human) RNF212 Antibody raised in Rabbit

Description

Definition and Function of RNF212 Antibody

The RNF212 antibody is a polyclonal or monoclonal antibody designed to detect and localize the RNF212 protein, a meiosis-specific E3 ubiquitin ligase essential for crossover regulation. It enables researchers to visualize RNF212's dynamic localization on synaptonemal complexes (SCs) during prophase I of meiosis and investigate its interactions with recombination machinery .

Key Applications in Research

Immunostaining:

Used to track RNF212's localization during meiotic stages in mouse spermatocytes and oocytes.
Revealed RNF212's association with early recombination sites and synaptonemal complexes (e.g., colocalization with SYCP1 and SYCP3) .

Immunoblotting:

Confirmed RNF212 protein expression levels in testis extracts, including its interdependence with paralog RNF212B for stability .

Protein Interaction Studies:

Validated physical interactions between RNF212, RNF212B, and HEI10 via yeast two-hybrid assays .

Dynamic Localization During Meiosis

Stage	RNF212 Localization Pattern	Key Insight
Zygonema	Foci at initial synaptonemal complex (SC) sites	Couples SC formation to crossover designation .
Pachynema	Reduced to 1–2 foci per SC, marking crossover precursors	Selective stabilization of MutSγ (MSH4-MSH5) at crossover sites .
Diplonema	Loss of foci precedes SC disassembly	Antagonized by HEI10, which promotes crossover resolution .

Functional Insights

Crossover Control: RNF212 stabilizes MutSγ complexes at recombination sites, enabling crossover formation. Loss of RNF212 abolishes crossovers .
Interdependence with RNF212B: Both proteins require mutual interaction for stability and chromosomal localization. Disruption of either reduces crossover rates .
Antagonism with HEI10: HEI10 (E3 ubiquitin ligase) counteracts RNF212 by promoting MutSγ removal, restricting crossovers to 1–2 per chromosome .

Technical Validation of the Antibody

Specificity: Confirmed via immunoblotting in Rnf212 and Rnf212b knockout mice, showing reduced protein levels .
Super-Resolution Imaging: Structured illumination microscopy (SIM) resolved RNF212’s precise localization along SCs .
Colocalization Studies: Demonstrated overlap with γH2AX (DNA damage marker) in Sycp1−/− mutants but not wild-type cells .

Implications for Meiosis Research

The RNF212 antibody has been pivotal in elucidating:

Crossover Designation: How specific recombination sites are selected for crossovers .
Protein Dynamics: Temporal regulation of recombination complexes (e.g., MutSγ stabilization vs. removal) .
Evolutionary Conservation: Functional parallels with S. cerevisiae Zip3 and C. elegans ZHP-3 .

Product Specs

Buffer

PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. Store at -20°C. Avoid freeze-thaw cycles.

Lead Time

Typically, we can ship your order within 1-3 business days of receiving it. Delivery times may vary depending on your location and the selected shipping method. For specific delivery information, please consult your local distributor.

Synonyms

FLJ38841 antibody; Hypothetical protein LOC285498 antibody; LOC285498 antibody; OTTHUMP00000147525 antibody; Probable E3 SUMO-protein ligase RNF212 antibody; RING finger protein 212 antibody; RN212_HUMAN antibody; RNF 212 antibody; RNF212 antibody; ZHP3 antibody; ZHP3; C. elegans; homolog of antibody; ZIP3-related protein antibody

Target Names

RNF212

Uniprot No.

Q495C1

Target Background

Function

RNF212 is a SUMO E3 ligase that plays a crucial role in regulating crossing-over during meiosis. It acts as a mediator in coupling chromosome synapsis with the formation of crossover-specific recombination complexes. RNF212 localizes to recombination sites and stabilizes meiosis-specific recombination factors, including proteins from the MutS-gamma complex (MSH4 and MSH5) and TEX11. It is believed that RNF212 might mediate the sumoylation of MSH4 and/or MSH5, enhancing their binding to recombination sites. This ligase acts as a limiting factor for crossover designation and/or reinforcement, and plays an antagonistic role with CCNB1IP1/HEI10 in the regulation of meiotic recombination.

Gene References Into Functions

A polymorphism in the RNF212 gene, specifically SNP rs4045481, may be linked to azoospermia. The CC genotype of this SNP might be a risk factor for azoospermia. PMID: 29277047
This publication describes a partial transcript of the human RNF212 gene. PMID: 15340062
Genome-wide searches identified sequence variants in the 4p16.3 region that correlate with recombination rates in both males and females. These variants are located in the RNF212 gene, a putative ortholog of the ZHP-3 gene, which is essential for recombination and chiasma formation in C. elegans. PMID: 18239089
This study provides observational data and a genome-wide association study of gene-disease associations. (HuGE Navigator) PMID: 18239089

Database Links

HGNC: 27729

OMIM: 612041

KEGG: hsa:285498

STRING: 9606.ENSP00000389709

UniGene: Hs.248290

Subcellular Location

Nucleus. Chromosome.

Quick Inquiry

Personal Email Detected

Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Name*

Email*

Phone

Country

Source

Quantity&Size*

Product Name

Message

RNF212 Antibody